Format

Send to

Choose Destination
Methods Mol Biol. 2019;1996:311-324. doi: 10.1007/978-1-4939-9488-5_23.

Protocols for NMR Analysis in Livestock Metabolomics.

Author information

1
Department of Biological Sciences, University of Alberta, Edmonton, AB, Canada.
2
Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, AB, Canada.
3
Department of Biological Sciences, University of Alberta, Edmonton, AB, Canada. dwishart@ualberta.ca.
4
Department of Computing Science, University of Alberta, Edmonton, AB, Canada. dwishart@ualberta.ca.

Abstract

Nuclear magnetic resonance (NMR) spectroscopy is widely considered to be one of the most robust and reproducible analytical platforms for conducting metabolomic experiments. As a metabolomic platform, NMR is not particularly sensitive, but it is nondestructive and requires no prior derivatization or chromatographic separation. It is also very automatable, easy to perform, and highly reproducible and can be used to accurately quantify dozens of metabolites in complex mixtures. To perform a successful NMR metabolomic experiment, it is important to follow good practices in sample preparation, data acquisition, and data analysis. In this chapter, we will describe, step-by-step, the preparation of different livestock samples, including both biofluids (whole blood, serum, urine, rumen content, and fecal water) and tissues (liver, muscle, testis). We will also describe the protocols for acquiring optimal NMR spectra and the techniques used to identify and quantify water-soluble metabolites by NMR spectroscopy.

KEYWORDS:

Biofluid; Livestock; Metabolomics; Nuclear magnetic resonance; Tissue; Water-soluble metabolites

Supplemental Content

Full text links

Icon for Springer
Loading ...
Support Center