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Res Vet Sci. 2019 Aug;125:24-35. doi: 10.1016/j.rvsc.2019.05.010. Epub 2019 May 15.

Hepatotoxicity and renal toxicity induced by gamma-radiation and the modulatory protective effect of Ficus carica in male albino rats.

Author information

1
Zoology Department, College of Science, King Saud University, Riyadh, Saudi Arabia; Zoology and Entomology Department, Faculty of Science, Helwan University, Ein Helwan, Helwan, Egypt. Electronic address: dibrahim@ksu.edu.sa.
2
Zoology Department, College of Science, King Saud University, Riyadh, Saudi Arabia.
3
Zoology Department, College of Science, King Saud University, Riyadh, Saudi Arabia; Zoology Department, Faculty of Science, Cairo University, Cairo, Egypt.
4
Biochemistry Department, College of Science, King Saud University, Riyadh, Saudi Arabia; Molecular Biology Department, Genetic Engineering Division, National Research Centre, Giza, Egypt.

Abstract

It is well-known that gamma radiation initiates generation of free radicals which prompting serious cellular damages in biological systems. In the present study, we investigated the role of Ficus carica, a natural antioxidant substance, in modulating changes in liver and kidney functions, antioxidant enzyme's gene expression, and apoptosis, in male albino rats exposed to gamma radiation. A total of 40 rats were used in this experiment and divided equally into 4 groups: Group 1, rats administered distilled H2O (Control); Group 2, rats administered F. carica; Group 3, rats irradiated; and Group 4, rats treated with F. carica and irradiated. Groups 3 and 4 were exposed to whole-body gamma radiations at a dose level of 8 Gy and with a dose rate of 0.762 Gy/min. F. carica was administered to rats by gavage, for 3 consecutive weeks, before exposure to radiation. Five rats were sacrificed from each group at intervals of 24 and 72 h after cessation of treatment. The results revealed marked increases in alanine aminotransferase and aspartate aminotransferase levels in liver, a decrease in albumin level and increase in urea level in kidney. Irradiation resulted in cytotoxic effects as indicated by elevation in antioxidant enzyme's gene expression at 24 h, the opposite was observed at 72 h. Immunohistochemical analysis revealed that cytochrome c and p53 expressions significantly increased following exposure to radiation. Oral administration of F. carica pre-irradiation as a natural product plays a modulatory protective and anti-apoptotic role against cells damaged by free radicals induced by whole-body irradiation.

KEYWORDS:

Anti-oxidant properties; Ficus carica; Hepatotoxicity; Radiation; Renal toxicity

PMID:
31125819
DOI:
10.1016/j.rvsc.2019.05.010

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