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Biomedicine (Taipei). 2019 Jun;9(2):12. doi: 10.1051/bmdcn/2019090212. Epub 2019 May 24.

Gynura bicolor aqueous extract attenuated H2O2 induced injury in PC12 cells.

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Department of Food Nutrition and Health Biotechnology, Asia University, Taichung 413, Taiwan.
Department of Food Nutrition and Health Biotechnology, Asia University, Taichung 413, Taiwan - Department of Medical Research, China Medical University Hospital, China Medical University, Taichung 404, Taiwan.



Protective effects of Gynura bicolor aqueous extract (GAE) at three concentrations upon nerve growth factor (NGF) differentiated-PC12 cells against H2O2 induced injury were examined.


NGF differentiated-PC12 cells were treated with GAE at 0.25%, 0.5% or 1%. 100 μM H2O2 was used to treat cells with GAE pre-treatments. After incubating at 37 °C for 12 hr, experimental analyses were processed.


H2O2 exposure decreased cell viability, increased plasma membrane damage, suppressed Bcl-2 mRNA expression and enhanced Bax mRNA expression. GAE pre-treatments reversed these changes. H2O2 exposure reduced mitochondrial membrane potential, lowered Na+-K+-ATPase activity, and increased DNA fragmentation and Ca2+ release. GAE pre-treatments attenuated these alterations. H2O2 stimulated the production of reactive oxygen species (ROS), interleukin (IL)-1beta, IL-6 and tumor necrosis factor-alpha, lowered glutathione content, and reduced glutathione peroxidase (GPX) and catalase activities. GAE pretreatments maintained GPX and catalase activities; and concentration-dependently diminished the generation of ROS and inflammatory cytokines. H2O2 enhanced mRNA expression of nuclear factor kappa (NF-κ) B and p38. GAE pre-treatments decreased mRNA expression of NF-κB and p38.


These findings suggested that GAE might be a potent neuronal protective agent.

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