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Iran J Parasitol. 2019 Jan-Mar;14(1):52-58.

Genotyping of Echinococcus granulosus Isolates from Human in Khorasan Province, North-Eastern Iran.

Author information

1
Department of Parasitology and Mycology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
2
Department of Thoracic Surgery, Cardiothoracic Surgery and Transplant Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.

Abstract

Background:

Human hydatidosis is endemic in northeastern Iran. The present study aimed to investigate molecular diversity of Echinococcus granulosus isolates collected from human surgically.

Methods:

Sixty human hydatid cysts (58 lung cysts and 2 liver cysts) were collected through surgery from Ghaem and Emam Reza hospitals in Mashhad University of Medical Sciences during 2015-2016. Cysts were characterized using polymerase chain reaction-restriction fragment length polymorphism (PCRRFLP) analysis of the internal transcribed spacer 1 (ITS1) gene and sequencing fragments of the genes coding for mitochondrial cytochrome c oxidase subunit 1 (cox1) and NADH dehydrogenase subunit I (nad1).

Results:

Overall, 55 out of 60 Echinococcus granulosus cysts (91.6%) were determined as the G1 strain, 4 cases (6.6%) were determined as the G6 strain and 1 sample was not identified.

Conclusion:

Although sheep strain (G1) is dominated in human patients in Great Khorasan, the prevention of camel-dog cycle should pay attention in this region.

KEYWORDS:

Echinococcus granulosus; Human; Hydatid; Iran; Strain

PMID:
31123468
PMCID:
PMC6511601

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