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Molecules. 2019 May 22;24(10). pii: E1967. doi: 10.3390/molecules24101967.

Functional Fragments of AIMP1-Derived Peptide (AdP) and Optimized Hydrosol for Their Topical Deposition by Box-Behnken Design.

Author information

1
College of Pharmacy, Chungnam National University, Daejeon 34134, Korea. daegeon0705@cnu.ac.kr.
2
College of Pharmacy, Chungnam National University, Daejeon 34134, Korea. gksdudals93@naver.com.
3
College of Pharmacy, Chungnam National University, Daejeon 34134, Korea. mds_wantae@naver.com.
4
College of Pharmacy, Chungnam National University, Daejeon 34134, Korea. gcy70426@gmail.com.
5
College of Pharmacy, Chungnam National University, Daejeon 34134, Korea. sol4273@naver.com.
6
College of Pharmacy, Chungnam National University, Daejeon 34134, Korea. woojin@cnu.ac.kr.
7
CureBio Research Center, Suwon 16229, Korea. jnkim@curebio.co.kr.
8
CureBio Research Center, Suwon 16229, Korea. sjkang@curebio.co.kr.
9
College of Pharmacy, Chungnam National University, Daejeon 34134, Korea. sangkim@cnu.ac.kr.
10
College of Pharmacy, Chungnam National University, Daejeon 34134, Korea. chocw@cnu.ac.kr.
11
Laboratory Animal Resource Center, Korea Research Institute of Bioscience and Biotechnology, Ochang 28116, Korea. kyeongrlee@kribb.re.kr.
12
College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University, Seoul 08826, Korea. ddkim@snu.ac.kr.
13
CureBio Research Center, Suwon 16229, Korea. mcpark@curebio.co.kr.
14
College of Pharmacy, Chungnam National University, Daejeon 34134, Korea. jaeyoung@cnu.ac.kr.

Abstract

Aminoacyl-tRNA synthetase complex-interacting multifunctional protein 1 (AIMP1)-derived peptide (AdP) has been developed as a cosmeceutical ingredient for skin anti-aging given its fibroblast-activating (FA) and melanocyte-inhibiting (MI) functions. However, a suitable strategy for the topical delivery of AdP was required due to its low-permeable properties. In this study, FA and MI domains of AdP (FA-AdP and MI-AdP, respectively) were determined by functional domain mapping, where the activities of several fragments of AdP on fibroblast and melanocyte were tested, and a hydrosol-based topical delivery system for these AdP fragments was prepared. The excipient composition of the hydrosol was optimized to maximize the viscosity and drying rate by using Box-Behnken design. The artificial skin deposition of FA-AdP-loaded hydrosol was evaluated using Keshary-Chien diffusion cells equipped with Strat-M membrane (STM). The quantification of the fluorescent dye-tagged FA-AdP in STM was carried out by near-infrared fluorescence imaging. The optimized hydrosol showed 127-fold higher peptide deposition in STM than free FA-AdP (p < 0.05). This work suggests that FA- and MI-AdP are active-domains for anti-wrinkle and whitening activities, respectively, and the hydrosol could be used as a promising cosmetic formulation for the delivery of AdPs to the skin.

KEYWORDS:

AIMP1-derived peptide (AdP); Box-Behnken design; cosmeceutical peptide; hydrosol; topical delivery of peptides

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