In this research, a core-shell immobilized lipase was constructed with the cost-effective bamboo carrier. Natural bamboo powder was pretreated by different methods. The results of SEM and BET demonstrated that alkali pretreatment increased the surface roughness with formed wrinkles and micropores, facilitating the lipase adsorption on the pretreated carrier. The optimal immobilization conditions were obtained through the response surface method, and the optimized conditions were 2 wt% sodium hydroxide solution, 1.2 wt% sodium alginate, and 0.69 wt% calcium chloride at a calcification time of 100 min. Under this condition, the immobilized lipase showed an excellent protein adsorption rate and thermal stability. When the core-shell structured immobilized lipase was used in wax ester synthesis system, the esterification yield (80%) did not show an obvious decline after 17 batches. The results indicated that the core-shell immobilized lipase is effective for the biocatalytic reaction, and the immobilization method is a promising strategy for future enzyme immobilization and modification in industrial applications.
Keywords: Bamboo; Calcium alginate; Candida sp. 99–125 lipase; Core–shell structure; Sodium alginate.