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Rev Peru Med Exp Salud Publica. 2019 Jan-Mar;36(1):46-53. doi: 10.17843/rpmesp.2019.361.3649. Epub 2019 May 13.

[Molecular characterization of the rabies virus' nucleoprotein in dogs from Arequipa, Peru].

[Article in Spanish; Abstract available in Spanish from the publisher]

Author information

1
Laboratorio de Zoonosis Virales, Instituto Nacional de Salud. Lima , Perú.
2
Laboratorio de Sistemática y Diversidad Vegetal, Museo de Historia Natural, Universidad Nacional Mayor de San Marcos. Lima, Perú.
3
Laboratorio de Bacteriología, Instituto Butantan. São Paulo, Brasil.
4
Programa de Posgrado Interunidades en Biotecnología. Universidade de São Paulo, Brasil.

Abstract

in English, Spanish

OBJECTIVE:

. To characterize the nucleoprotein (N) and establish the origin of the rabies virus in dogs coming from Arequipa.

MATERIALS AND METHODS.:

Thirty samples of nervous tissue from the departments of Arequipa and Puno were analyzed. Total RNA was extracted from the samples and cDNA was synthesized to amplify the nucleoprotein gene, sequence it, and perform bioinformatics analysis.

RESULTS:

. A defined group was formed with respect to the external group (European bat lyssavirus). This group was classified into two subgroups, one constituted by samples coming from Puno and Arequipa (subgroup A), and another one by samples from Puno (subgroup B), exhibiting a nucleotide identity of 99.9% in subgroup A. This group was classified in two subgroups, one constituted by samples coming from Puno and Arequipa (subgroup A), and another one by samples from Puno (subgroup B), observing a nucleotide identity of 99.9% in subgroup A.

CONCLUSIONS.:

The groupings of viral sequences show that the cases of canine rabies reported in Arequipa are the result of the expansion of canine rabies from the endemic region of Puno.

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