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PLoS Biol. 2019 May 21;17(5):e3000251. doi: 10.1371/journal.pbio.3000251. eCollection 2019 May.

A 3D-printed hand-powered centrifuge for molecular biology.

Author information

1
Lambert High School, Suwanee, Georgia, United States of America.
2
School of Chemical & Biomolecular Engineering, Georgia Institute of Technology, Atlanta, Georgia, United States of America.
3
Department of Integrative Biology, University of California, Berkeley, California, United States of America.

Abstract

The centrifuge is an essential tool for many aspects of research and medical diagnostics. However, conventional centrifuges are often inaccessible outside of standard laboratory settings, such as remote field sites, because they require a constant external power source and can be prohibitively costly in resource-limited settings and Science, technology, engineering, and mathematics (STEM)-focused programs. Here we present the 3D-Fuge, a 3D-printed hand-powered centrifuge, as a novel alternative to standard benchtop centrifuges. Based on the design principles of a paper-based centrifuge, this 3D-printed instrument increases the volume capacity to 2 mL and can reach hand-powered centrifugation speeds up to 6,000 rpm. The 3D-Fuge devices presented here are capable of centrifugation of a wide variety of different solutions such as spinning down samples for biomarker applications and performing nucleotide extractions as part of a portable molecular lab setup. We introduce the design and proof-of-principle trials that demonstrate the utility of low-cost 3D-printed centrifuges for use in remote field biology and educational settings.

PMID:
31112539
PMCID:
PMC6528969
DOI:
10.1371/journal.pbio.3000251
[Indexed for MEDLINE]
Free PMC Article

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