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J Exp Bot. 2019 Apr 24. pii: erz189. doi: 10.1093/jxb/erz189. [Epub ahead of print]

Transcript and Metabolite Changes during the Early Phase of ABA-mediated Induction of CAM in Talinum triangulare.

Author information

1
Institute of Plant Biochemistry, Cluster of Excellence on Plant Sciences (CEPLAS), Heinrich-Heine-University, Düsseldorf, Germany.

Abstract

Crassulacean Acid Metabolism (CAM) has evolved as a water saving strategy and its engineering into crops offers an opportunity to improve their water-use efficiency. This requires a comprehensive understanding of the regulation of the CAM pathway. Here, we use the facultative CAM species Talinum triangulare as a model in which CAM can be induced rapidly by exogenous abscisic acid (ABA). RNA-sequencing and metabolite measurements were employed to analyse the changes underlying CAM induction and identify potential CAM regulators. Non-negative matrix factorisation followed by k -means clustering identified an early CAM-specific cluster and a late one, which was specific for the early light phase. Enrichment analysis revealed ABA metabolism, WRKY-regulated transcription, sugar and nutrient transport and protein degradation in these clusters. Activation of the CAM pathway was supported by up-regulation of phosphoenolpyruvate carboxylase, cytosolic and chloroplastic malic enzymes and several transport proteins as well as by increased end-of-night titratable acidity and malate accumulation. Transcription factors HSFA2, NF-YA9 and JMJ27 were identified as candidate regulators of CAM induction. With this study we promote the model species T. triangulare, in which CAM can be induced in a controlled way, enabling further deciphering of CAM regulation.

KEYWORDS:

Talinum triangulare ; abscisic acid; crassulacean acid metabolism; metabolome; time course; transcriptome

PMID:
31111894
DOI:
10.1093/jxb/erz189

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