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Nat Cell Biol. 2019 Jun;21(6):721-730. doi: 10.1038/s41556-019-0330-5. Epub 2019 May 20.

EGFR is required for Wnt9a-Fzd9b signalling specificity in haematopoietic stem cells.

Author information

1
Department of Cellular and Molecular Medicine, University of California, San Diego, La Jolla, CA, USA.
2
Biomedical Sciences Graduate Program, University of California, San Diego, La Jolla, CA, USA.
3
Skaggs School of Pharmacy and Pharmaceutical Science, University of California, San Diego, La Jolla, CA, USA.
4
Department of Pharmacology, University of California, San Diego, La Jolla, CA, USA.
5
Massachusetts General Hospital Nephrology Division, Charlestown, MA, USA.
6
Harvard Medical School, Department of Genetics, Boston, MA, USA.
7
Department of Cellular and Molecular Medicine, University of California, San Diego, La Jolla, CA, USA. kwillert@ucsd.edu.
8
Department of Cellular and Molecular Medicine, University of California, San Diego, La Jolla, CA, USA. dtraver@ucsd.edu.
9
Section of Cell and Developmental Biology, University of California, San Diego, La Jolla, CA, USA. dtraver@ucsd.edu.

Abstract

Wnt signalling drives many processes in development, homeostasis and disease; however, the role and mechanism of individual ligand-receptor (Wnt-Frizzled (Fzd)) interactions in specific biological processes remain poorly understood. Wnt9a is specifically required for the amplification of blood progenitor cells during development. Using genetic studies in zebrafish and human embryonic stem cells, paired with in vitro cell biology and biochemistry, we determined that Wnt9a signals specifically through Fzd9b to elicit β-catenin-dependent Wnt signalling that regulates haematopoietic stem and progenitor cell emergence. We demonstrate that the epidermal growth factor receptor (EGFR) is required as a cofactor for Wnt9a-Fzd9b signalling. EGFR-mediated phosphorylation of one tyrosine residue on the Fzd9b intracellular tail in response to Wnt9a promotes internalization of the Wnt9a-Fzd9b-LRP signalosome and subsequent signal transduction. These findings provide mechanistic insights for specific Wnt-Fzd signals, which will be crucial for specific therapeutic targeting and regenerative medicine.

PMID:
31110287
PMCID:
PMC6559346
[Available on 2019-11-20]
DOI:
10.1038/s41556-019-0330-5

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