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Plant Physiol. 2019 May 20. pii: pp.00124.2019. doi: 10.1104/pp.19.00124. [Epub ahead of print]

NPR1 promotes its own and target gene expression in plant defense by recruiting CDK8.

Author information

1
University of South Carolina CITY: Columbia STATE: South Carolina United States Of America [US].
2
Duke University CITY: Durham United States Of America [US].
3
Nanjing Agricultural University CITY: Nanjing China [CN].
4
University of Edinburgh CITY: Edinburgh POSTAL_CODE: EH9 3BF United Kingdom [GB].
5
Purdue University CITY: West Lafayette United States Of America [US].
6
Institute of Genetics and Developmental Biology, Chinese Academy of Sciences Datun Road, Chaoyang District CITY: Beijing POSTAL_CODE: 100101 China [CN].
7
Jiangsu Academy of Agricultural Sciences CITY: Nanjing China [CN].
8
University of South Carolina CITY: Columbia STATE: South Carolina POSTAL_CODE: 29208 United States Of America [US] ZFU@mailbox.sc.edu.

Abstract

NPR1 (NONEXPRESSER OF PR GENES 1) functions as a master regulator of the plant hormone salicylic acid (SA) signaling and plays an essential role in plant immunity. In the nucleus, NPR1 interacts with transcription factors to induce the expression of PR (Pathogenesis-Related) genes and thereby promote defense responses. However, the underlying molecular mechanism of PR gene activation is poorly understood. Furthermore, despite the importance of NPR1 in plant immunity, the regulation of NPR1 expression has not been extensively studied. Here, we show that SA promotes the interaction of NPR1 with both CDK8 (cyclin-dependent kinase 8) and WRKY18 (WRKY DNA-binding protein 18) in Arabidopsis thaliana. NPR1 recruits CDK8 and WRKY18 to the NPR1 promoter, facilitating its own expression. Intriguingly, CDK8 and its associated Mediator subunits positively regulate NPR1 and PR1 expression and play a pivotal role in local and systemic immunity. Moreover, CDK8 interacts with WRKY6, WRKY18, and TGA transcription factors and brings RNA polymerase II to NPR1 and PR1 promoters and coding regions to facilitate their expression. Our studies reveal a mechanism in which NPR1 recruits CDK8, WRKY18 and TGA transcription factors along with RNA polymerase II in the presence of SA and thereby facilitates its own and target gene expression for the establishment of plant immunity.

PMID:
31110139
DOI:
10.1104/pp.19.00124

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