miR-145-5p Acts as a Novel Tumor Suppressor in Hepatocellular Carcinoma Through Targeting RAB18

Technol Cancer Res Treat. 2019 Jan 1:18:1533033819850189. doi: 10.1177/1533033819850189.

Abstract

Micro-RNAs play critical roles in initiation and progression of hepatocellular carcinoma. However, the biological role of microRNA-145-5p in hepatocellular carcinoma and how it works are still not clearly understood. Expression levels of microRNA-145-5p in hepatocellular carcinoma cell lines were examined by reverse transcription quantitative polymerase chain reaction. Cell counting kit-8, wound-healing assay, and flow cytometry assay were conducted to investigate the role of microRNA-145-5p von proliferation, migration, and apoptosis. Luciferase reporter assay and Western blot were performed to investigate the correlation between microRNA-145-5p and RAB18. We found microRNA-145-5p was downregulated in hepatocellular carcinoma cell lines compared to the normal cell line. Overexpression of microRNA-145-5p inhibited the proliferation and migration but promoted apoptosis of hepatocellular carcinoma cells in vitro. RAB18 was validated a target of microRNA-145-5p and ectopic expression of RAB18 can promote the proliferation and migration but inhibit apoptosis of hepatocellular carcinoma cells. These findings indicate that microRNA-145-5p functions as a novel tumor suppressor through targeting RAB18, suggesting that microRNA-145-5p might be a potential new therapeutic molecule for the treatment of hepatocellular carcinoma.

Keywords: RAB18; cell behaviors; hepatocellular carcinoma; miR-145-5p; tumor suppressor.

Publication types

  • Retracted Publication

MeSH terms

  • 3' Untranslated Regions
  • Apoptosis / genetics
  • Carcinoma, Hepatocellular / genetics*
  • Cell Line, Tumor
  • Cell Movement / genetics
  • Cell Proliferation
  • Gene Expression Regulation, Neoplastic*
  • Genes, Tumor Suppressor*
  • Humans
  • Liver Neoplasms / genetics*
  • MicroRNAs / genetics*
  • RNA Interference*
  • rab GTP-Binding Proteins / genetics*

Substances

  • 3' Untranslated Regions
  • MIRN145 microRNA, human
  • MicroRNAs
  • RAB18 protein, human
  • rab GTP-Binding Proteins