Send to

Choose Destination
Front Immunol. 2019 Apr 24;10:717. doi: 10.3389/fimmu.2019.00717. eCollection 2019.

Envelope-Specific Recognition Patterns of HIV Vaccine-Induced IgG Antibodies Are Linked to Immunogen Structure and Sequence.

Author information

Division of Infectious Diseases and Tropical Medicine, University Hospital, LMU Munich, Munich, Germany.
German Center for Infection Research (DZIF), Partner Site Munich, Munich, Germany.
MRC Clinical Trials Unit at UCL, London, United Kingdom.
Institute of Medical Microbiology and Hygiene, University Regensburg, Regensburg, Germany.
NIMR-Mbeya Medical Research Center, Mbeya, Tanzania.
Muhimbili University of Health and Allied Sciences, Dar es Salaam, Tanzania.
JPT Peptide Technologies, Berlin, Germany.
Department of Medicine, Imperial College London, London, United Kingdom.
US Military HIV Research Program, Silver Spring, MD, United States.
Department of Clinical Science and Education, Karolinska Institutet at Södersjukhuset, Stockholm, Sweden.
Institute of Clinical Microbiology and Hygiene, University Hospital, Regensburg, Germany.
Institute of Global Health (CIMI), University of Liverpool, Liverpool, United Kingdom.


Background: A better understanding of the parameters influencing vaccine-induced IgG recognition of individual antigenic regions and their variants within the HIV Envelope protein (Env) can help to improve design of preventive HIV vaccines. Methods: Env-specific IgG responses were mapped in samples of the UKHVC003 Standard Group (UK003SG, n = 11 from UK) and TaMoVac01 (TMV01, n = 17 from Tanzania) HIV vaccine trials. Both trials consisted of three immunizations with DNA, followed by two boosts with recombinant Modified Vaccinia Virus Ankara (MVA), either mediating secretion of gp120 (UK003SG) or the presentation of cell membrane bound gp150 envelopes (TMV01) from infected cells, and an additional two boosts with 5 μg of CN54gp140 protein adjuvanted with glucopyranosyl lipid adjuvant (GLA). Env immunogen sequences in UK003SG were solely based on the clade C isolate CN54, whereas in TMV01 these were based on clades A, C, B, and CRF01AE. The peptide microarray included 8 globally representative Env sequences, CN54gp140 and the MVA-encoded Env immunogens from both trials, as well as additional peptide variants for hot spots of immune recognition. Results: After the second MVA boost, UK003SG vaccinees almost exclusively targeted linear, non-glycosylated antigenic regions located in the inter-gp120 interface. In contrast, TMV01 recipients most strongly targeted the V2 region and an immunodominant region in gp41. The V3 region was frequently targeted in both trials, with a higher recognition magnitude for diverse antigenic variants observed in the UK003SG (p < 0.0001). After boosting with CN54gp140/GLA, the overall response magnitude increased with a more comparable recognition pattern of antigenic regions and variants between the two trials. Recognition of most immunodominant regions within gp120 remained significantly stronger in UK003SG, whereas V2-region recognition was not boosted in either group. Conclusions: IgG recognition of linear antigenic Env regions differed between the two trials particularly after the second MVA boost. Structural features of the MVA-encoded immunogens, such as secreted, monomeric gp120 vs. membrane-anchored, functional gp150, and differences in prime-boost immunogen sequence variability most probably contributed to these differences. Prime-boosting with multivalent Env immunogens during TMV01 did not improve variant cross-recognition of immunodominant peptide variants in the V3 region.


HIV; envelope-specific antibodies; epitope variant recognition; immunogen sequence; immunogen structure; vaccine

Supplemental Content

Full text links

Icon for Frontiers Media SA Icon for PubMed Central
Loading ...
Support Center