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Fish Physiol Biochem. 2019 Jun;45(3):921-933. doi: 10.1007/s10695-018-0596-8. Epub 2019 May 19.

Three forms of cocaine- and amphetamine-regulated transcript may be involved in food intake regulation in gibel carp (Carassius auratus gibelio).

Author information

1
Department of Aquaculture, College of Animal Science, Southwest University, Chongqing, 402460, People's Republic of China.
2
Tibet Academy of Agricultural and Animal Husbandry Sciences, Lhasa, 850002, Tibet, People's Republic of China.
3
Department of Aquaculture, College of Life Sciences, Neijiang Normal University, Neijiang, 641000, Sichuan, People's Republic of China.
4
Tibet Academy of Agricultural and Animal Husbandry Sciences, Lhasa, 850002, Tibet, People's Republic of China. lbh0891@163.com.
5
Department of Aquaculture, College of Animal Science, Southwest University, Chongqing, 402460, People's Republic of China. zhengzonglin@126.com.

Abstract

In fish, as in mammals, several studies have demonstrated that the cocaine- and amphetamine-regulated transcript (CART) plays an important role in feeding. However, thus far, the function of CART in gibel carp (Carassius auratus gibelio) feeding regulation has not been reported. In our study, we first identified three forms of CART peptide precursors from gibel carp brain and named these CART-1, CART-2, and CART-3. The full-length cDNA sequences of CART-1, CART-2, and CART-3 were 616 bp, 705 bp, and 760 bp, respectively, encoding peptides of 118, 120, and 104 amino acid residues. We detected mRNA expression of CART-1, CART-2, and CART-3 in a wide range of peripheral and central tissues, with the highest expression detected in the brain. After a meal, mRNA expression of CART-1, CART-2, and CART-3 was significantly elevated, suggesting that CART-1, CART-2, and CART-3 may act as postprandial satiety signals. Moreover, mRNA expression of all three CART-1, CART-2, and CART-3 was significantly reduced during fasting and significantly elevated with refeeding. Our findings indicate that CART-1, CART-2, and CART-3 might function as a satiety factor in the gibel carp.

KEYWORDS:

Cloning; Cocaine- and amphetamine-regulated transcript; Fasting; Feeding; Gibel carp

PMID:
31104250
DOI:
10.1007/s10695-018-0596-8

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