Format

Send to

Choose Destination
Appl Microbiol Biotechnol. 2019 May 17. doi: 10.1007/s00253-019-09801-3. [Epub ahead of print]

Peptide dendrimers G3KL and TNS18 inhibit Pseudomonas aeruginosa biofilms.

Author information

1
The Key Laboratory of Molecular Microbiology and Technology Ministry of Education, Nankai University, Tianjin, 300071, China.
2
Electricity Information and Automation College, Civil Aviation University of China, Tianjin, 300300, China.
3
Key Laboratory of Systems Biology, State Key Laboratory of Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, 200031, China.
4
Department of Chemistry and Biochemistry, University of Bern, 3012, Bern, Switzerland.
5
Department of Chemistry and Biochemistry, University of Bern, 3012, Bern, Switzerland. jean-louis.reymond@dcb.unibe.ch.
6
The Key Laboratory of Molecular Microbiology and Technology Ministry of Education, Nankai University, Tianjin, 300071, China. mingqiangqiao@aliyun.com.

Abstract

Herein we report that peptide dendrimers G3KL and TNS18, which were recently reported to control multidrug-resistant bacteria such as Staphylococcus aureus, Pseudomonas aeruginosa, and Acinetobacter baumannii, strongly inhibit biofilm formation by P. aeruginosa PA14 below their minimum inhibitory concentration (MIC) value, under which conditions they also strongly affect swarming motility. Eradication of preformed biofilms, however, required concentrations above the MIC values. Scanning electron microscopy observation and confocal laser scanning micrographs showed that peptide dendrimers can destroy the biofilm morphological structure and thickness in a dose-dependent manner, even make the biofilm dispersed completely. Membrane potential analysis indicated that planktonic cells treated with peptide dendrimers presented an increase in fluorescence intensity, suggesting that cytoplasmic membrane could be the target of G3KL and TNS18 similarly to polymyxin B. RNA-seq analysis showed that the expressions of genes in the arnBCADTEF operon-regulating lipid A modification resulting in resistance to AMPs are differentially affected between these three compounds, suggesting that each compound targets the cell membrane but in different manner. Potent activity on planktonic cells and biofilms of P. aeruginosa suggests that peptide dendrimers G3KL and TNS18 are promising candidates of clinical development for treating infections.

KEYWORDS:

Biofilm; Membrane; Mingqiang Qiao and Jean-Louis Reymond contributed equally to this work.; Peptide dendrimers; Polymyxin B; Pseudomonas aeruginosa

PMID:
31101943
DOI:
10.1007/s00253-019-09801-3

Supplemental Content

Full text links

Icon for Springer
Loading ...
Support Center