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Toxicol In Vitro. 2019 Oct;60:76-86. doi: 10.1016/j.tiv.2019.05.010. Epub 2019 May 14.

Anhydroglucitol-core gallotannins from red maple buds modulate viability of human blood neutrophils.

Author information

1
Département des Sciences du Bois et de la Forêt, Faculté de Foresterie et Géomatique, Centre de Recherche sur les Matériaux Renouvelables (CRMR), Université Laval, 2425 rue de la Terrasse, Pavillon G-H Kruger, Québec, QC G1V 0A6, Canada; Institut des Nutraceutiques et des Aliments Fonctionnels (INAF), Université Laval, 2440 Hochelaga Blvd, Québec, QC G1V 0A6, Canada; Centre de Recherche sur les Matériaux Avancés (CERMA), Université Laval, 1065 avenue de la médecine, Québec, QC G1V 0A6, Canada; Département de Médecine, Centre de Recherche en Rhumatologie et Immunologie, Centre de Recherche du CHU de Québec, Université Laval, 2705 Boulevard Laurier, Québec, QC G1V 4G2, Canada.
2
Département des Sciences du Bois et de la Forêt, Faculté de Foresterie et Géomatique, Centre de Recherche sur les Matériaux Renouvelables (CRMR), Université Laval, 2425 rue de la Terrasse, Pavillon G-H Kruger, Québec, QC G1V 0A6, Canada; Institut des Nutraceutiques et des Aliments Fonctionnels (INAF), Université Laval, 2440 Hochelaga Blvd, Québec, QC G1V 0A6, Canada; Centre de Recherche sur les Matériaux Avancés (CERMA), Université Laval, 1065 avenue de la médecine, Québec, QC G1V 0A6, Canada.
3
Département de Médecine, Centre de Recherche en Rhumatologie et Immunologie, Centre de Recherche du CHU de Québec, Université Laval, 2705 Boulevard Laurier, Québec, QC G1V 4G2, Canada. Electronic address: patrice.poubelle@crchudequebec.ulaval.ca.

Abstract

Apoptosis of neutrophils is an essential checkpoint for the resolution of inflammation by shutting down the deleterious functions of these immune cells. This study investigated the role of anhydroglucitol-core gallotannins (ACGs) in apoptosis increase of human blood neutrophils treated by the hot water extract from red maple buds (RMB). Fractions obtained by liquid-liquid partitioning (ethyl acetate, butanol and water-remaining fractions) of the hot water extract from RMB were assessed for their effects on neutrophil viability by using flow cytometry. These fractions were then phytochemically analyzed to investigate the ability of major compounds to induce neutrophil apoptosis individually. Ethyl acetate and butanol fractions that contained the major ACGs ginnalin A, ginnalin 3,6 and ginnalin C stimulated the apoptosis of neutrophils. The three ACGs at 100 μM significantly increased the rate of the late apoptotic cells. When differentially combined, these ACGs have additive or antagonist effects. These effects are related to the concentrations of the constituents in the mixtures studied, especially so for ginnalin C. GinA increased FADD, phospho-Rad17, SMAC/Diablo and cytochrome C, while decreasing the anti-apoptotic protein catalase. These compounds could be useful for the development of novel therapeutic approaches that facilitate resolution of neutrophil-mediated inflammatory diseases.

KEYWORDS:

Acer rubrum L.; Anhydroglucitol-core gallotannins; Apoptosis; Buds; Neutrophils; Red maple

PMID:
31100377
DOI:
10.1016/j.tiv.2019.05.010

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