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Mol Biol (Mosk). 2019 Mar-Apr;53(2):349-352. doi: 10.1134/S0026898419020046.

[Protein Biosynthesis Proofreading Is Closely Associated with the Existence of Factor-Free Ribosomal Synthesis].

[Article in Russian]

Author information

1
Institute of Protein Research, Russian Academy of Sciences, Pushchino, Moscow oblast, 142290 Russia.
2
Faculty of Biology, Moscow State University, Moscow, 119991 Russia.
3
afinkel@vega.protres.ru.

Abstract

Despite protein biosynthesis being studied for decades, some major questions concerning this process are still to be addressed. We elucidate a close connection between proofreading of the emerging amino acid sequence during its normal, elongation factor-dependent ribosomal biosynthesis and the existence of the factor-free synthesis of a polypeptide chain on a ribosome. In this factor-free process, the biological role of proofreading is played by a process opposite to the factor-free attachment of Aa-tRNA to the ribosome, namely, the removal via the same pathway of that Aa-tRNA, which is not complementary to the mRNA codon exhibited by the ribosome.

KEYWORDS:

GTP; biosynthesis of polypeptides; codon recognition; elongation factor Tu; factor-free process; free energy; parallel reactions; proofreading; ribosome

PMID:
31099785
DOI:
10.1134/S0026898419020046
[Indexed for MEDLINE]
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