Solubilization of protein BM-40 from a basement membrane tumor with chelating agents and evidence for its identity with osteonectin and SPARC

FEBS Lett. 1987 Jun 22;218(1):167-72. doi: 10.1016/0014-5793(87)81040-2.

Abstract

Up to 80% of the calcium-binding protein BM-40 could be extracted from a tumor basement membrane with a physiological buffer containing 10 mM EDTA. About half of its amino acid sequence was determined by Edman degradation demonstrating identity with cDNA deduced sequences of bone osteonectin and SPARC.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Basement Membrane / analysis*
  • Basement Membrane / drug effects
  • Calcium-Binding Proteins / isolation & purification*
  • Carrier Proteins / genetics
  • Carrier Proteins / isolation & purification*
  • DNA / genetics
  • Edetic Acid / pharmacology
  • Mice
  • Neoplasm Proteins / isolation & purification
  • Neoplasms, Experimental / analysis
  • Osteonectin

Substances

  • Calcium-Binding Proteins
  • Carrier Proteins
  • Neoplasm Proteins
  • Osteonectin
  • DNA
  • Edetic Acid