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Nat Commun. 2019 May 16;10(1):2183. doi: 10.1038/s41467-019-10201-4.

The Pseudomonas aeruginosa lectin LecB binds to the exopolysaccharide Psl and stabilizes the biofilm matrix.

Author information

1
Department of Microbiology, University of Washington, Seattle, WA, USA.
2
Istituto di Cristallografia, Consiglio Nazionale delle Ricerche, Sede Secondaria di Basovizza, Trieste, Italy.
3
Departments of Microbial Infection and Immunity, Microbiology, Ohio State University, Columbus, OH, USA.
4
Department of Microbiology, University of Washington, Seattle, WA, USA. parsem@uw.edu.
5
Integrative Microbiology Research Centre, South China Agricultural University, 510642, Guangzhou, China. parsem@uw.edu.

Abstract

Pseudomonas aeruginosa biofilms are composed of exopolysaccharides (EPS), exogenous DNA, and proteins that hold these communities together. P. aeruginosa produces lectins LecA and LecB, which possess affinities towards sugars found in matrix EPS and mediate adherence of P. aeruginosa to target host cells. Here, we demonstrate that LecB binds to Psl, a key matrix EPS, and this leads to increased retention of both cells and EPS in a growing biofilm. This interaction is predicted to occur between the lectin and the branched side chains present on Psl. Finally, we show that LecB coordinates Psl localization in the biofilm. This constitutes a unique function for LecB and identifies it as a matrix protein that contributes to biofilm structure through EPS interactions.

PMID:
31097723
PMCID:
PMC6522473
DOI:
10.1038/s41467-019-10201-4
[Indexed for MEDLINE]
Free PMC Article

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