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BMC Genomics. 2019 May 16;20(1):381. doi: 10.1186/s12864-019-5737-7.

Comparative proteomic analysis of okra (Abelmoschus esculentus L.) seedlings under salt stress.

Author information

1
State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou, 310058, China.
2
Institute of Agricultural Equipment, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China.
3
Institute of Horticulture, Zhejiang Academy of Agriculture Science, Hangzhou, 310021, China.
4
The Key Laboratory for Quality Improvement of Agricultural Products of Zhejiang Province, School of Agriculture and Food Science, Zhejiang A&F University, Linan, Hangzhou, 311300, China.
5
College of Modern Science and Technology, China Jiliang University, Hangzhou, 310018, China.
6
Institute of Agricultural Equipment, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China. 21007030@zju.edu.cn.

Abstract

BACKGROUND:

Salinization seriously threatens land use efficiency and crop yields across the world. Understanding the mechanisms plants use to protect against salt stress will help breeders develop salt-tolerant vegetable crops. Okra (Abelmoschus esculentus L.) is an important vegetable crop of the mallow family, which is now cultivated in warm regions worldwide. To understand the effects of salt stress on the protein level of okra, a comparative proteomic analysis of okra seedlings grown in the presence of 0 or 300 mmol L- 1 NaCl treatment was performed using an integrated approach of Tandem Mass Tag labeling and LC-MS/MS integrated approach.

RESULTS:

A total of 7179 proteins were identified in this study, for which quantitative information was available for 5774 proteins. In the NaCl/control comparison group, there were 317 differentially expressed proteins (DEPs), of which 165 proteins were upregulated and 152 proteins downregulated in the presence of NaCl. Based on the above data, we carried out a systematic bioinformatics analysis of proteins with information, including protein annotation, domain characteristics, functional classification, and pathway enrichment. Enriched gene ontology and Kyoto Encyclopedia of Genes and Genomes pathway analysis showed that the DEPs were most strongly associated with "response to stress" and "protein processing in endoplasmic reticulum". Furthermore, several heat shock proteins were identified as DEPs.

CONCLUSIONS:

This information provides a reference direction for further research on the okra proteome in the downstream of the salt stress response, with our data revealing that the responses of okra to salt stress involves by various pathways.

KEYWORDS:

Differential expression protein; Heat shock proteins; Okra; Proteomics; Salt stress; TMT labeling

PMID:
31096913
PMCID:
PMC6521433
DOI:
10.1186/s12864-019-5737-7
[Indexed for MEDLINE]
Free PMC Article

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