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Anticancer Res. 2019 May;39(5):2561-2567. doi: 10.21873/anticanres.13378.

Association Between Formalin Fixation Time and Programmed Cell Death Ligand 1 Expression in Patients With Non-Small Cell Lung Cancer.

Author information

1
Department of Respiratory Medicine, Kobe City Medical Center General Hospital, Kobe, Japan.
2
Department of Respiratory Medicine, Kobe City Medical Center General Hospital, Kobe, Japan daichi@kcho.jp.
3
Department of Pathology, Kobe City Medical Center General Hospital, Kobe, Japan.
4
Department of Pathology, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan.
5
Department of Thoracic Surgery, Kobe City Medical Center General Hospital, Kobe, Japan.

Abstract

BACKGROUND/AIM:

The expression of programmed cell death ligand 1 (PD-L1) determined by immunohistochemistry (IHC) may be associated with tissue formalin fixation time in non-small cell lung cancer (NSCLC) samples. We investigated the association between the PD-L1 expression and formalin fixation time, and clarified the optimal duration of fixation for accurate PD-L1 evaluation.

MATERIALS AND METHODS:

We collected 55 tumor specimens from resected NSCLC patients. The samples were halved and immediately fixed in 10% buffered formalin for 12-24 h (normal fixation), or 96-120 h (prolonged fixation). Each specimen was stained using two assay systems (22C3 and SP263) for PD-L1.

RESULTS:

The mean PD-L1 tumor proportion score was not significantly different between normal and prolonged fixation groups for either 22C3 or SP263 (normal fixation: 18.8%; prolonged fixation: 16.3%, p=0.277; normal fixation: 16.2%; prolonged fixation: 17.6%, p=0.560, respectively).

CONCLUSION:

Formalin fixation duration for up to 120 h does not affect PD-L1 IHC expression. PD-L1 tumor proportion score of tumor specimens can be evaluated by IHC even if these have been fixed in formalin outside the recommended duration in clinical practice.

KEYWORDS:

22C3; PD-1; PD-L1; SP263; fixation time; formalin fixation; immunohistochemistry

PMID:
31092453
DOI:
10.21873/anticanres.13378
[Indexed for MEDLINE]

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