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J Proteome Res. 2019 Aug 2;18(8):3020-3031. doi: 10.1021/acs.jproteome.9b00142. Epub 2019 May 22.

Fabric Phase Sorptive Extraction-A Metabolomic Preprocessing Approach for Ionizing Radiation Exposure Assessment.

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Lerner Research Institute , Cleveland Clinic Foundation , 9500 Euclid Avenue , Cleveland , Ohio 44195 , United States.
International Forensic Research Institute, Department of Chemistry and Biochemistry , Florida International University , 11200 Southwest Eighth Street , Miami , Florida 33199 , United States.
Center for Applied NanoBiosience and Medicine , University of Arizona , 475 North Fifth Street , Phoenix , Arizona 85004 , United States.
Center for Radiological Research , Columbia University , 630 West 168th Street , New York , New York 10032 , United States.
Translational Genomics Research Institute , 445 North Fifth Street , Phoenix , Arizona 85004 , United States.
Department of Basic Medical Sciences , College of Medicine Phoenix , 425 North Fifth Street , Phoenix , Arizona 85004 , United States.


The modern application of mass spectrometry-based metabolomics to the field of radiation assessment and biodosimetry has allowed for the development of prompt biomarker screenings for radiation exposure. Our previous work on radiation assessment, in easily accessible biofluids (such as urine, blood, saliva), has revealed unique metabolic perturbations in response to radiation quality, dose, and dose rate. Nevertheless, the employment of swift injury assessment in the case of a radiological disaster still remains a challenge as current sample processing can be time consuming and cause sample degradation. To address these concerns, we report a metabolomics workflow using a mass spectrometry-compatible fabric phase sorptive extraction (FPSE) technique. FPSE employs a matrix coated with sol-gel poly(caprolactone-b-dimethylsiloxane-b-caprolactone) that binds both polar and nonpolar metabolites in whole blood, eliminating serum processing steps. We confirm that the FPSE preparation technique combined with liquid chromatography-mass spectrometry can distinguish radiation exposure markers such as taurine, carnitine, arachidonic acid, α-linolenic acid, and oleic acid found 24 h after 8 Gy irradiation. We also note the effect of different membrane fibers on both metabolite extraction efficiency and the temporal stabilization of metabolites in whole blood at room temperature. These findings suggest that the FPSE approach could work in future technology to triage irradiated individuals accurately, via biomarker screening, by providing a novel method to stabilize biofluids between collection and sample analysis.


biodosimetry; extraction; fabric phase sorptive extraction; metabolite stability; metabolomics; radiation; untargeted metabolomics; whole blood extraction

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