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Huan Jing Ke Xue. 2019 May 8;40(5):2349-2356. doi: 10.13227/j.hjkx.201811106.

[Treatment of Piggery Biogas Slurry by Enhanced Biological Contact Oxidation with HN-AD Bacteria].

[Article in Chinese]

Author information

1
School of Chemical Engineering, Chongqing University of Technology, Chongqing 400054, China.
2
Chongqing Academy of Environmental Science, Chongqing 401147, China.

Abstract

The conventional pretreatment process for swine wastewater is anaerobic fermentation. This process leads to the formation of high ammonia nitrogen, low carbon, and piggery biogas slurry, which usually results in poor denitrification effect, complicated process flow, and long startup period for the subsequent treatment process. In this study, a novel biological enhanced Biological Contact Oxidation (BCO) process using HN-AD bacteria as microbial inoculants, and PAN activated carbon fiber filler as biofilm carrier was proposed for the treatment of piggery biogas slurry. In the early stage of sludge acclimation, it was found that when NH4+-N concentration was higher than 500 mg·L-1, the nitrification and COD removal in BCO was severely inhibited. When the BCO was enhanced by HN-AD bacteria, however, the tolerance concentration of NH4+-N for bacteria in BCO could reach 600 mg·L-1 and the removal efficiency of NH4+-N, COD, and TN could still remain at a high level. The bio-enhanced BCO process was used to treat the piggery biogas slurry. The average removal rates of NH4+-N, TN, and COD were 86.9%, 70.5%, and 74.4%, respectively, which were higher than the 57.6%, 50.3%, and 50.0% of the traditional treatment process. The concentration of the pollutants mentioned above in the effluent was below the relevant discharge standards. The changes in the microbial community structure during the enrichment process of functional bacteria were studied by high-throughput sequencing technique. The results showed that the dominant bacteria belonging to HN-AD in the biofilm during the sludge acclimation process was Alcaligenes. After the addition of the HN-AD agent, however, the dominant bacteria were Diaphorobacter, Acinetobacter, and Thauer, and the relative abundance of Acinetobacter was much higher than that in the microbial inoculants. The results of scanning electron microscopy further confirmed the existence of bio-enhancement. The surface of the biofilm layer tightly attached to the filler was enriched with rod-like and globular HN-AD functional bacteria.

KEYWORDS:

HN-AD agent; biological contact oxidation (BCO)process; biological enhancement; microbial community structure; piggery biogas slurry

PMID:
31087876
DOI:
10.13227/j.hjkx.201811106

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