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Bioessays. 2019 Jun;41(6):e1900031. doi: 10.1002/bies.201900031. Epub 2019 May 14.

Efficient and Innocuous Live-Cell Delivery: Making Membrane Barriers Disappear to Enable Cellular Biochemistry: How Better Cellular Delivery Tools Can Contribute to Precise and Quantitative Cell Biology Assays.

Author information

1
Department of Biochemistry and Biophysics, Texas A&M University, College Station, Bldg, room 436, 300 Olsen Blvd, TX, 77843-2128, USA.
2
Department of Chemistry, Texas A&M University, College Station, TX, USA.

Abstract

The confluence of protein engineering techniques and delivery protocols are providing new opportunities in cell biology. In particular, techniques that render the membrane of cells transiently permeable make the introduction of nongenetically encodable macromolecular probes into cells possible. This, in turn, can enable the monitoring of intracellular processes in ways that can be both precise and quantitative, ushering an area that one may envision as cellular biochemistry. Herein, the author reviews pioneering examples of such new cell-based assays, provides evidence that challenges the paradigm that cell penetration is a necessarily damaging and stressful event for cells, and highlights some of the challenges that should be addressed to fully unlock the potential of this nascent field.

KEYWORDS:

cell biochemistry; cell biology; cell delivery

PMID:
31087674
PMCID:
PMC6563813
[Available on 2020-06-01]
DOI:
10.1002/bies.201900031

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