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CNS Neurosci Ther. 2019 May 13. doi: 10.1111/cns.13149. [Epub ahead of print]

Transplantation of Nurr1-overexpressing neural stem cells and microglia for treating parkinsonian rats.

Author information

1
Yunnan Key Laboratory of Laboratory Medicine, Yunnan Engineering Technology Center of Digestive disease, 1st Affiliated Hospital of Kunming Medical University, Kunming, China.
2
Genetic Diagnosis Center, Women and Children Hospital, Kunming, China.
3
Department of Neurosurgery, 1st Affiliated Hospital of Kunming Medical University, Kunming, China.
4
Department of Neurosurgery, The Central Hospital of Wenzhou, Wenzhou, China.
5
Institute of Neuroscience, Kunming Medical University, Kunming, China.
6
Rehabilitation Engineering Research Laboratory, Biomedicine Engineering Research Centre, Kunming Medical University, Kunming, China.
7
Department of Anatomy, Kunming Medical University, Kunming, China.
8
The Key Laboratory of Stem Cell and Regenerative Medicine of Yunnan Province, Institute of Molecular and Clinical Medicine, Kunming Medical University, Kunming, China.

Abstract

BACKGROUND:

Neural stem cells (NSCs) transplantation is considered a promising treatment for Parkinson's disease. But most NSCs are differentiated into glial cells rather than neurons, and only a few of them survive after transplantation due to the inflammatory environment.

METHODS:

In this study, neural stem cells (NSCs) and microglial cells both forced with the Nurr1 gene were transplanted into the striatum of the rat model of PD. The results were evaluated through reverse transcription polymerase chain reaction (RT-PCR), Western blot, and immunofluorescence analysis.

RESULTS:

The behavioral abnormalities of PD rats were improved by combined transplantation of NSCs and microglia, both forced with Nurr1. The number of tyrosine hydroxylase+ cells in the striatum of PD rats increased, and the number of Iba1+ cells decreased compared with the other groups. Moreover, the dopamine neurons differentiated from grafted NSCs could still be detected in the striatum of PD rats after 5 months.

CONCLUSIONS:

The results suggested that transplantation of Nurr1-overexpressing NSCs and microglia could improve the inhospitable host brain environments, which will be  a new potential strategy for the cell replacement therapy in PD.

KEYWORDS:

Parkinson's disease; inflammatory; microglia; neural stem cells; nuclear receptor-related factor 1

PMID:
31087449
DOI:
10.1111/cns.13149

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