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Clin Chem Lab Med. 2019 Sep 25;57(10):1511-1521. doi: 10.1515/cclm-2019-0214.

Pre-analytical practices for routine coagulation tests in European laboratories. A collaborative study from the European Organisation for External Quality Assurance Providers in Laboratory Medicine (EQALM).

Author information

1
Department of Medical Biochemistry and Pharmacology, Haukeland University Hospital, Bergen, Norway.
2
Norwegian Quality Improvement of Laboratory Examinations (Noklus), Haraldsplass Deaconess Hospital, Bergen, Norway.
3
Central Laboratory, Jósa University Hospital, Nyíregyháza, Hungary.
4
Department of Global Public Health and Primary Care, University of Bergen, Bergen, Norway.
5
Quality of laboratories, Sciensano, Brussels, Belgium.
6
UK NEQAS for Blood Coagulation, Sheffield, UK.
7
Sheffield Haemophilia and Thrombosis Centre, Sheffield, UK.
8
Quality Control Center Switzerland (CSCQ), Chêne-Bourg, Switzerland.
9
ECAT foundation, Voorschoten, The Netherlands.

Abstract

Background Correct handling and storage of blood samples for coagulation tests are important to assure correct diagnosis and monitoring. The aim of this study was to assess the pre-analytical practices for routine coagulation testing in European laboratories. Methods In 2013-2014, European laboratories were invited to fill in a questionnaire addressing pre-analytical requirements regarding tube fill volume, citrate concentration, sample stability, centrifugation and storage conditions for routine coagulation testing (activated partial thromboplastin time [APTT], prothrombin time in seconds [PT-sec] and as international normalised ratio [PT-INR] and fibrinogen). Results A total of 662 laboratories from 28 different countries responded. The recommended 3.2% (105-109 mmol/L) citrate tubes are used by 74% of the laboratories. Tube fill volumes ≥90% were required by 73%-76% of the laboratories, depending upon the coagulation test and tube size. The variation in centrifugation force and duration was large (median 2500 g [10- and 90-percentiles 1500 and 4000] and 10 min [5 and 15], respectively). Large variations were also seen in the accepted storage time for different tests and sample materials, for example, for citrated blood at room temperature the accepted storage time ranged from 0.5-72 h and 0.5-189 h for PT-INR and fibrinogen, respectively. If the storage time or the tube fill requirements are not fulfilled, 72% and 84% of the respondents, respectively, would reject the samples. Conclusions There was a large variation in pre-analytical practices for routine coagulation testing in European laboratories, especially for centrifugation conditions and storage time requirements.

KEYWORDS:

activated partial thromboplastin time; coagulation tests; fibrinogen; haemostasis; pre-analytical phase; prothrombin time

PMID:
31085743
DOI:
10.1515/cclm-2019-0214

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