Format

Send to

Choose Destination
J Gen Physiol. 2019 Jul 1;151(7):929-943. doi: 10.1085/jgp.201912333. Epub 2019 May 13.

Extraocular muscle function is impaired in ryr3 -/- mice.

Author information

1
Department of Anesthesia, Basel University Hospital, Basel, Switzerland.
2
Department of Biomedicine, Basel University Hospital, Basel, Switzerland.
3
Department of Ophthalmology, University Hospital of Bern, Bern, Switzerland.
4
Department of Biomedical Research, University of Bern, Bern, Switzerland.
5
Department of Surgery, Davis Heart & Lung Research Institute, The Ohio State University Medical Center, Columbus, OH.
6
Graduate School of Pharmaceutical Sciences, Kyoto University, Kyoto, Japan.
7
Department of Life Sciences, Microbiology and Applied Pathology section, University of Ferrara, Ferrara, Italy.
8
Department of Anesthesia, Basel University Hospital, Basel, Switzerland susan.treves@unibas.ch.
#
Contributed equally

Abstract

Calcium is an ubiquitous second messenger mediating numerous physiological processes, including muscle contraction and neuronal excitability. Ca2+ is stored in the ER/SR and is released into the cytoplasm via the opening of intracellular inositol trisphosphate receptor and ryanodine receptor calcium channels. Whereas in skeletal muscle, isoform 1 of the RYR is the main channel mediating calcium release from the SR leading to muscle contraction, the function of ubiquitously expressed ryanodine receptor 3 (RYR3) is far from clear; it is not known whether RYR3 plays a role in excitation-contraction coupling. We recently reported that human extraocular muscles express high levels of RYR3, suggesting that such muscles may be useful to study the function of this isoform of the Ca2+ channel. In the present investigation, we characterize the visual function of ryr3-/- mice. We observe that ablation of RYR3 affects both mechanical properties and calcium homeostasis in extraocular muscles. These changes significantly impact vision. Our results reveal for the first time an important role for RYR3 in extraocular muscle function.

PMID:
31085573
PMCID:
PMC6605690
[Available on 2020-01-01]
DOI:
10.1085/jgp.201912333

Supplemental Content

Full text links

Icon for HighWire
Loading ...
Support Center