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Mol Cell Probes. 2019 Jun;45:65-67. doi: 10.1016/j.mcp.2019.05.001. Epub 2019 May 10.

Development of a loop-mediated isothermal amplification assay for Vibrio cholerae O1 and O139.

Author information

1
Department of Bacteriology I, National Institute of Infectious Diseases, Tokyo, Japan. Electronic address: izumiya@nih.go.jp.
2
Department of Bacteriology I, National Institute of Infectious Diseases, Tokyo, Japan.
3
Department of Bacteriology, National Institute for Hygiene and Epidemiology, Hanoi, Viet Nam.

Abstract

A loop-mediated isothermal amplification assay was developed. It was designed for recognizing Vibrio cholerae O1/O139, where atpA, rfbN, and wfbR genes were adopted. The assay specifically detected the target with sensitivities of 5-67 copies per reaction in 1 h. The assay will aid rapid detection of the cholera bacterium.

KEYWORDS:

Cholera; LAMP; O1/O139

PMID:
31082474
DOI:
10.1016/j.mcp.2019.05.001

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