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Int J Biol Macromol. 2019 May 10;134:695-703. doi: 10.1016/j.ijbiomac.2019.05.061. [Epub ahead of print]

Development of a reliable microRNA based electrochemical genosensor for monitoring of miR-146a, as key regulatory agent of neurodegenerative disease.

Author information

1
Stem Cell Research Center (SCRC), Tabriz University of Medical Sciences, Tabriz, Iran; Biosensors and Bioelectronics Research Center, Ardabil University of Medical Sciences, Ardabil, Iran. Electronic address: khalilzadehb@tbzmed.ac.ir.
2
Research Center for Pharmaceutical Nanotechnology (RCPN), Tabriz University of Medical Sciences, Tabriz, Iran.
3
Department of Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran.
4
Biophotonic Research Center, Islamic Azad University, Tabriz Branch, Tabtiz, Iran.
5
Department of Chemistry, Faculty of Sciences, Van Yuzuncu Yil University, 65080, Van, Turkey.
6
Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
7
Research Center for Pharmaceutical Nanotechnology (RCPN), Tabriz University of Medical Sciences, Tabriz, Iran; Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran.

Abstract

A MicroRNA (miR) based electrochemical method for quantification of miR-146a, a known biomarker for neurodegenerative disease, was developed. In this bioassay, the capture microRNA (C-miR) was self-assembled on the gold surface and used for quantification of target microRNA (T-miR) of miR-146a. For this purpose, an optimized concentration of C-miR was immobilized on the surface of gold electrode and used for capture of target analyte (T-miR). All of preparation steps were characterized by electrochemical techniques (cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS)) and atomic force microscopy (AFM). At the optimized conditions, the linear dynamic range, limit of quantification and relative standard deviation of the proposed bioassay were obtained as 10 pM to 1 μM, 10 pM and 1.59%, respectively. The unprocessed human serum sample was used as a real sample and the results fully confirm that the designed microRNA based biosensor is capable for detection of miR-146a as neurodegenerative disease biomarker. The developed method offers a more precise and high sensitive tool to be used in clinical applications for early detection of neurodegenerative disease like Alzheimer's and Parkinson.

KEYWORDS:

Alzheimer; Biomedical analysis; Biosensing; Neurodegenerative; RNA; miR-146a

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