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Biomed Res Int. 2019 Apr 8;2019:5068258. doi: 10.1155/2019/5068258. eCollection 2019.

High Glucose Enhances the Odonto/Osteogenic Differentiation of Stem Cells from Apical Papilla via NF-KappaB Signaling Pathway.

Wang Y1,2, Wang Y2,3, Lu Y2,4, Yu J2,3.

Author information

1
Endodontic Department, the Affiliated Stomatological Hospital of Soochow University, Suzhou Stomatological Hospital, 1505 Renmin Road, Suzhou, Jiangsu 215005, China.
2
Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, 136 Hanzhong Road, Nanjing, Jiangsu 210029, China.
3
Endodontic Department, School of Stomatology, Nanjing Medical University, 136 Hanzhong Road, Nanjing, Jiangsu 210029, China.
4
Endodontic Department of the West Branch of Hangzhou Dental Hospital, 306 Tianmushan Road, Hangzhou, Zhejiang 310013, China.

Abstract

Objective:

The transport and metabolism of glucose are important during mammalian development. High glucose can mediate the biological characteristics of mesenchymal stem cells (MSCs). However, the role of high glucose in the odonto/osteogenic differentiation of stem cells from apical papilla (SCAPs) is unclear.

Materials and Methods:

SCAPs were isolated and identified in vitro. Then, SCAPs were cultured in normal α-MEM and high glucose α-MEM separately. MTT assay was applied to observe the proliferation of SCAPs. ALP activity, alizarin red staining, real-time RT-PCR, and western blot were used to detect the odonto/osteogenic capacity of SCAPs as well as the participation of NF-κB pathway.

Results:

SCAPs in 25mmol/L glucose group expressed the maximum proteins of RUNX2 and ALP as compared with those in 5, 10, and 15 mmol/L groups. MTT assay showed that 25 mmol/L glucose suppressed the proliferation of SCAPs. ALP assay, alizarin red staining, real-time RT-PCR, and western blot showed 25 mmol/L high glucose can obviously enhance the odonto/osteogenic capacity of SCAPs. Moreover, the NF-κB pathway was activated in 25mmol/L glucose-treated SCAPs and the odonto/osteogenic differentiation was inhibited following the inhibition of NF-κB signaling pathway.

Conclusions:

High glucose can enhance the odonto/osteogenic capacity of SCAPs via NF-κB pathway.

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