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Curr Neuropharmacol. 2019 Apr 9. doi: 10.2174/1570159X17666190409143155. [Epub ahead of print]

Using Cell Cultures for the Investigation of Treatments for Attention Deficit Hyperactivity Disorder: Systematic Review.

Author information

1
Department of Pharmacy, Universidade Estadual de Maringá, Maringá. Brazil.
2
Department of Medical and Pharmaceutical Sciences, Universidade Estadual do Oeste do Paraná, Cascavel. Brazil.
3
Department of Pharmacy, Universidade Federal do Paraná, Curitiba. Brazil.
4
Department of Social Pharmacy, Faculty of Pharmacy, Universidade de Lisboa, Lisbon. Portugal.

Abstract

BACKGROUND:

Advances in basic and molecular biology have promoted the use of cell cultures in a wide range of areas, including for the evaluation of drug efficacy, safety and toxicity.

OBJECTIVE:

This article was to provide a general overview of the methodological parameters of cell cultures used to investigate therapeutic options for Attention Deficit Hyperactivity Disorder.

METHOD:

A systematic search was performed in the electronic databases PubMed, Scopus, and DOAJ. In vitro experimental studies using cell cultures were included.

RESULTS:

A total of 328 studies were initially identified, with 16 included for qualitative synthesis. Seven studies used neuronal cells (SH-SY5Y neuroblastoma and PC12 cell line) and nine used non-neuronal cells. All studies described the culture conditions, but most studies were inconsistent with regard to reporting results and raw data. Only one-third of the studies performed cell viability assays, while a further 30% conducted gene expression analysis. Other additional tests included electrophysiological evaluation and transporter activity. More than 50% of the studies evaluated the effects of drugs such as methylphenidate and atomoxetine, while plant extracts were assessed in four studies and polyunsaturated fatty acids in one.

CONCLUSION:

We suggested a flowchart to guide the planning and execution of studies, and a checklist to be completed by authors to allow the standardized reporting of results. This may guide the elaboration of laboratory protocols and further in vitro studies.

KEYWORDS:

ADHD; ADHD treatment; cell model; checklist.; flowchart; methodological aspect; neuronal cell

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