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Andrology. 2020 Jan;8(1):191-200. doi: 10.1111/andr.12648. Epub 2019 May 12.

In vitro effect of autologous platelet-rich plasma on H2 O2 -induced oxidative stress in human spermatozoa.

Author information

1
Mount Lebanon Hospital, Azoury IVF Clinic, Beirut, Lebanon.
2
Faculty of Medicine, Regenerative Medicine and Inflammation Laboratory, Saint-Joseph University, Beirut, Lebanon.
3
Faculty of Public Health II, Medical Laboratory Department, Lebanese University, Beirut, Lebanon.
4
Department of Obstetrics and Gynecology, American University of Beirut Medical Center, Beirut, Lebanon.
5
OB-GYN Department, Inova Fairfax Hospital, Falls Church, VA, USA.
6
Faculty of Medical Sciences, Neuroscience Research Center, Lebanese University, Beirut, Lebanon.

Abstract

BACKGROUND:

Among new therapies emerging in the medical field, the use of platelet-rich plasma (PRP) in human reproduction has not yet been explored.

OBJECTIVES:

This study aimed at investigating the effect of autologous PRP on sperm parameters in the presence and absence of H2 O2 .

MATERIALS AND METHODS:

Semen samples were collected from 30 healthy men in their fourth decade attending Azoury IVF clinic. Spermatozoa, cultured in the presence or absence of 10 μM H2 O2 , were left untreated or treated with increasing concentrations of PRP (2, 5, and 10%). After 24-h incubation, ROS levels were assessed and sperm parameters were evaluated.

RESULTS:

Our results highlight the harmful effect of H2 O2 on sperm parameters, showing an increase in the percentage of reactive oxygen species (ROS)-positive cells, vacuolization, and sperm DNA fragmentation, and a decrease in progressive and total motility in the H2 O2 -treated group compared to non-stressed spermatozoa. When samples were treated with PRP, an improvement of the studied parameters was noted mainly with 2% PRP, thus regarded as the best concentration to achieve a positive effect on sperm parameters. Indeed, non-stressed and stressed spermatozoa treated with 2% PRP showed a significant increase in progressive and total motility, coupled with a decrease in ROS-positive cells, DNA fragmentation, vacuolization, and dead cells compared to the untreated group. In contrast, no significant difference in cell morphology was found between the two groups. Moreover, 2% PRP treatment enhanced sperm parameters and prevented cell death in H2 O2 -exposed spermatozoa as compared to freshly collected semen.

DISCUSSION:

We suggest that PRP because of its wide arrays of growth factors included in his alpha granules contributes to the inhibition of ROS through the antioxidant, anti-apoptotic activity.

CONCLUSION:

Autologous PRP improves the quality of the sperm, more so in the presence of an H2 O2 -induced OS.

KEYWORDS:

male infertility; oxidative stress; platelet-rich plasma; spermatozoa

PMID:
31079423
DOI:
10.1111/andr.12648

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