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Neurosci Lett. 2019 Jul 27;706:51-55. doi: 10.1016/j.neulet.2019.05.010. Epub 2019 May 9.

Intravascular injections of adenoassociated viral vector serotypes rh10 and PHP.B transduce murine sciatic nerve axons.

Author information

1
Department of Bioengineering, University of Colorado - Anschutz Medical Campus, Aurora, CO, USA. Electronic address: hans.anderson@ucdenver.edu.
2
Department of Neurology, University of Colorado - Anschutz Medical Campus, Aurora, CO, USA.
3
Department of Cell and Developmental Biology, University of Colorado - Anschutz Medical Campus, Aurora, CO, USA.
4
Department of Bioengineering, University of Colorado - Anschutz Medical Campus, Aurora, CO, USA.

Abstract

Adenoassociated viral vectors provide a safe and robust method for expression of transgenes in nondividing cells such as neurons. Intravenous injections of these vectors provide a means of transducing motoneurons of peripheral nerves. Previous research has demonstrated that serotypes 1, rh10 and PHP.B can transduce motor neuron cell bodies in the spinal cord, but has not quantified expression in the peripheral nerve axon. Axonal labeling is crucial for optogenetic stimulation and detection of action potentials in peripheral nerve. Therefore, in this study, serotypes 1, PHP.B, and rh10 were tested for their ability to label axons of the murine sciatic and tibial nerve following intravenous injection. Serotype rh10 elicits expression in 10% of acetylcholine transferase positive axons of the sciatic nerve in immunohistochemically-stained sections. Serotype rh10 transduces a variety of axon diameters from <1-12 μm, while PHP.B transduces larger axons of diameter (4-16 μm). Expression was not seen with serotype 1. These results show the potential of serotypes PHP.B and rh10 delivery of transgenic products to axons of the peripheral nerve.

KEYWORDS:

Adenoassociated viral vector; Green fluorescent protein; Intravascular injection; Motor neuron; Mouse; Peripheral nerve

PMID:
31078676
PMCID:
PMC6606055
[Available on 2020-07-27]
DOI:
10.1016/j.neulet.2019.05.010

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