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Stem Cell Res. 2019 May;37:101448. doi: 10.1016/j.scr.2019.101448. Epub 2019 May 4.

Generation of four patient-specific pluripotent induced stem cell lines from two Brazilian patients with amyotrophic lateral sclerosis and two healthy subjects.

Author information

1
Instituto de Biofísica Carlos Chagas Filho, UFRJ, Rio de Janeiro, Brazil; Instituto Nacional de Ciência e Tecnologia em Medicina Regenerativa, Rio de Janeiro, Brazil; Instituto de Ciências Biomédicas, Rio de Janeiro, Brazil. Electronic address: fegubert@biof.ufrj.br.
2
Instituto de Biofísica Carlos Chagas Filho, UFRJ, Rio de Janeiro, Brazil; Instituto Nacional de Ciência e Tecnologia em Medicina Regenerativa, Rio de Janeiro, Brazil. Electronic address: julianavasques@biof.ufrj.br.
3
Instituto de Biofísica Carlos Chagas Filho, UFRJ, Rio de Janeiro, Brazil; Instituto Nacional de Ciência e Tecnologia em Medicina Regenerativa, Rio de Janeiro, Brazil; Instituto Oswaldo Cruz/FIOCRUZ, Rio de Janeiro, Brazil.
4
Instituto de Biofísica Carlos Chagas Filho, UFRJ, Rio de Janeiro, Brazil; Instituto Nacional de Ciência e Tecnologia em Medicina Regenerativa, Rio de Janeiro, Brazil.
5
Instituto de Biofísica Carlos Chagas Filho, UFRJ, Rio de Janeiro, Brazil; Centro Nacional de Biologia Estrutural e Bioimagem - CENABIO, UFRJ, Rio de Janeiro, Brazil.
6
Instituto de Neurologia Deolindo Couto, UFRJ, Rio de Janeiro, Brazil.
7
Instituto Oswaldo Cruz/FIOCRUZ, Rio de Janeiro, Brazil.
8
Laboratório de Citogenética do Núcleo de Tecnologia Celular da Pontifícia Universidade Católica do Paraná, Curitiba, Brazil.
9
Instituto de Biofísica Carlos Chagas Filho, UFRJ, Rio de Janeiro, Brazil.

Abstract

Induced pluripotent stem cell (iPSC) lines were generated from erythroblasts of two patients with amyotrophic lateral sclerosis (ALS) and two healthy individuals. One familial and one sporadic ALS patients were used, both with genetic alterations in VAPB gene. CytoTune™-iPS 2.0 Sendai Reprogramming Kit (containing the reprogramming factors OCT3/4, KLF4, SOX2 and cMYC) was used to generate the iPSC cell lines. The four iPSCs express pluripotency markers, have normal karyotype and differentiated spontaneously in the three germ layers. The expression of Sendai virus was lost in all iPSC lines after 15 passages.

PMID:
31077962
DOI:
10.1016/j.scr.2019.101448
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