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Biomol NMR Assign. 2019 May 10. doi: 10.1007/s12104-019-09898-7. [Epub ahead of print]

Backbone resonance assignments and secondary structure of Ebola nucleoprotein 600-739 construct.

Author information

1
National Magnetic Resonance Facility at Madison, and Biochemistry Department, University of Wisconsin-Madison, 433 Babcock Drive, Madison, WI, 53706, USA.
2
Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, MO, 63110, USA.
3
National Magnetic Resonance Facility at Madison, and Biochemistry Department, University of Wisconsin-Madison, 433 Babcock Drive, Madison, WI, 53706, USA. jmarkley@wisc.edu.

Abstract

Ebola viral infections have resulted in several deadly epidemics in recent years in West and Central Africa. Because only one of the seven proteins encoded by the viral genome possesses enzymatic activity, disruption of protein-protein interactions is a promising route for antiviral drug development. We carried out a screening campaign to identify small, drug-like compounds that bind to the C-terminal region of the multifunctional Ebola nucleoprotein (eNP) with the objective of discovering ones that disrupt its binding to other Ebola proteins or to the single-stranded RNA genome. In the course of this effort we assigned the backbone 1H, 15N, and 13C resonances of residues 600‒739, the region that contains the critical eVP30 binding region 600‒615 targeted by host factors, and used the assigned chemical shifts to predict secondary structural features and peptide dynamics. This work supports and extends the previous X-ray crystal structures and NMR studies of residues 641‒739. We found that the 600‒739 domain consists of separate regions that are largely disordered and ordered.

KEYWORDS:

Ebola virus; Non-segmented negative-stranded RNA virus; Nucleoprotein; Virus replication

PMID:
31076990
DOI:
10.1007/s12104-019-09898-7

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