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Gut. 2019 May 10. pii: gutjnl-2018-317424. doi: 10.1136/gutjnl-2018-317424. [Epub ahead of print]

Development of resistance to FAK inhibition in pancreatic cancer is linked to stromal depletion.

Author information

1
Department of Medicine, Washington University School of Medicine, St. Louis, MO, USA.
2
Department of Biotherapy, State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, Chengdu, Sichuan, China.
3
Siteman Cancer Center, Washington University School of Medicine, St. Louis, MO, USA.
4
Department of Surgery, Washington University School of Medicine, St. Louis, MO, USA.
5
Verastem Inc., Needham, MA, USA.
6
Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, MO, USA.
#
Contributed equally

Abstract

OBJECTIVE:

We investigated how pancreatic cancer developed resistance to focal adhesion kinase (FAK) inhibition over time.

DESIGN:

Pancreatic ductal adenocarcinoma (PDAC) tumours from KPC mice (p48-CRE; LSL-KRasG12D/wt; p53flox/wt) treated with FAK inhibitor were analysed for the activation of a compensatory survival pathway in resistant tumours. We identified pathways involved in the regulation of signal transducer and activator of transcription 3 (STAT3) signalling on FAK inhibition by gene set enrichment analysis and verified these outcomes by RNA interference studies. We also tested combinatorial approaches targeting FAK and STAT3 in syngeneic transplantable mouse models of PDAC and KPC mice.

RESULTS:

In KPC mice, the expression levels of phosphorylated STAT3 (pSTAT3) were increased in PDAC cells as they progressed on FAK inhibitor therapy. This progression corresponded to decreased collagen density, lowered numbers of SMA+ fibroblasts and downregulation of the transforming growth factor beta (TGF-β)/SMAD signalling pathway in FAK inhibitor-treated PDAC tumours. Furthermore, TGF-β production by fibroblasts in vitro drives repression of STAT3 signalling and enhanced responsiveness to FAK inhibitor therapy. Knockdown of SMAD3 in pancreatic cancer cells abolished the inhibitory effects of TGF-β on pSTAT3. We further found that tumour-intrinsic STAT3 regulates the durability of the antiproliferative activity of FAK inhibitor, and combinatorial targeting of FAK and Janus kinase/STAT3 act synergistically to suppress pancreatic cancer progression in mouse models.

CONCLUSION:

Stromal depletion by FAK inhibitor therapy leads to eventual treatment resistance through the activation of STAT3 signalling. These data suggest that, similar to tumour-targeted therapies, resistance mechanisms to therapies targeting stromal desmoplasia may be critical to treatment durability.

KEYWORDS:

TGF-beta; drug resistance; myofibroblasts; pancreatic cancer; pancreatic fibrosis

PMID:
31076405
DOI:
10.1136/gutjnl-2018-317424

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