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New Phytol. 2019 May 10. doi: 10.1111/nph.15904. [Epub ahead of print]

The inconspicuous gatekeeper: endophytic Serendipita vermifera acts as extended plant protection barrier in the rhizosphere.

Author information

1
University of Cologne, Botanical Institute, Cluster of Excellence on Plant Sciences (CEPLAS), 50674, Cologne, Germany.
2
Institute of Plant Biochemistry, Department of Cell and Metabolic Biology, 06120, Halle (Saale), Germany.
3
University of Cologne, Institute of Zoology, Terrestrial Ecology, Cluster of Excellence on Plant Sciences (CEPLAS), 50674, Cologne, Germany.

Abstract

In nature, beneficial and pathogenic fungi often simultaneously colonize plants. Despite substantial efforts to understand the composition of natural plant-microbe communities, the mechanisms driving such multipartite interactions remain largely unknown. Here we address how the interaction between the beneficial root endophyte Serendipita vermifera and the pathogen Bipolaris sorokiniana affects fungal behavior and determines barley host responses using a gnotobiotic soil-based split-root system. Fungal confrontation in soil resulted in induction of B. sorokiniana genes involved in secondary metabolism and a significant repression of genes encoding putative effectors. In S. vermifera, genes encoding hydrolytic enzymes were strongly induced. This antagonistic response was not activated during the tripartite interaction in barley roots. Instead, we observed a specific induction of S. vermifera genes involved in detoxification and redox homeostasis. Pathogen infection but not endophyte colonization resulted in substantial host transcriptional reprogramming and activation of defense. In the presence of S. vermifera, pathogen infection and disease symptoms were significantly reduced despite no marked alterations of the plant transcriptional response. The activation of stress response genes and concomitant repression of putative effector gene expression in B. sorokiniana during confrontation with the endophyte suggest a reduction of the pathogen's virulence potential prior to host plant infection. This article is protected by copyright. All rights reserved.

KEYWORDS:

antagonism; diterpenoid metabolism; host defense; microbiota; phytoalexin

PMID:
31074884
DOI:
10.1111/nph.15904

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