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Anal Chem. 2019 Jun 4;91(11):6986-6990. doi: 10.1021/acs.analchem.9b01694. Epub 2019 May 17.

High-Precision Quantitation of Biofluid Samples Using Direct Mass Spectrometry Analysis.

Zhang W1,2, Ren Y3, Lin Z3, Ouyang Z1,2,3.

Author information

1
State Key Laboratory of Precision Measurement Technology and Instruments, Department of Precision Instrument , Tsinghua University , Beijing 100084 , China.
2
Department of Chemistry , Purdue University , West Lafayette , Indiana 47907 , United States.
3
Weldon School of Biomedical Engineering , Purdue University , West Lafayette , Indiana 47907 , United States.

Abstract

The transition of mass spectrometry for clinical analysis is highly desirable, and major progress has been made with direct sampling ionization for operation simplification. High-precision quantitation, however, remains a major challenge in this transition. Herein, a novel method was developed for direct quantitation of biofluid samples, using an extremely simplified procedure for incorporation of internal standards selected against the traditional rules. Slug flow microextraction was used for the development, with conditions predicted by a theoretical model, viz., using internal standards of partition coefficients very different from the analytes and large sample-to-extraction solvent volume ratios. Direct quantitation of drug compounds in urine and blood samples was demonstrated. This development enabled an extremely simplified protocol that is expected to have a significant impact on on-site or clinical analysis.

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