A dynamic, ultra-sensitive and "turn-on" strategy for fluorescent detection of uranyl based on DNAzyme and entropy-driven amplification initiated circular cleavage amplification

Wen Yun; Hong Wu; Zhehan Yang; Ruiqi Wang; Chongjun Wang; Lizhu Yang; Yongjian Tang

doi:10.1016/j.aca.2019.04.018

A dynamic, ultra-sensitive and "turn-on" strategy for fluorescent detection of uranyl based on DNAzyme and entropy-driven amplification initiated circular cleavage amplification

Anal Chim Acta. 2019 Aug 30:1068:104-110. doi: 10.1016/j.aca.2019.04.018. Epub 2019 Apr 12.

Authors

Wen Yun¹, Hong Wu², Zhehan Yang², Ruiqi Wang³, Chongjun Wang², Lizhu Yang⁴, Yongjian Tang⁵

Affiliations

¹ Chongqing Key Laboratory of Catalysis and New Environmental Materials, College of Environment and Resources, Chongqing Technology and Business University, Chongqing, 400067, China; State Key Laboratory of Environment-Friendly Energy Material, Southwest University of Science and Technology, Mianyang 621010, PR China. Electronic address: 44863542@qq.com.
² Chongqing Key Laboratory of Catalysis and New Environmental Materials, College of Environment and Resources, Chongqing Technology and Business University, Chongqing, 400067, China.
³ Chongqing Key Laboratory of Catalysis and New Environmental Materials, College of Environment and Resources, Chongqing Technology and Business University, Chongqing, 400067, China. Electronic address: wrqchem@qq.com.
⁴ School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou, Zhejiang, 325035, China. Electronic address: yanglz3000@aliyun.com.
⁵ State Key Laboratory of Environment-Friendly Energy Material, Southwest University of Science and Technology, Mianyang 621010, PR China.

PMID: 31072470
DOI: 10.1016/j.aca.2019.04.018

Abstract

A uranyl detection strategy with ultra-sensitivity was developed based on entropy-driven amplification and DNAzyme circular cleavage amplification. The cleavage of UO₂²⁺-specific DNAzyme produces a DNA fragment to initiate the entropy-driven amplification. Two DNA sequences released from the entropy-driven amplification are partly complementary. They can form an entire enzyme strand (E-DNA) of Mg²⁺-specific DNAzyme. The formed E-DNA can circularly cleave FAM-labeled probes on gold nanoparticles (AuNPs), causing the leaving of FAM from AuNPs and recovery of fluorescent signal. A linear relationship was obtained in the range from 30 pM to 5 nM between fluorescence intensity and concentration of UO₂²⁺. The limit of detection was low to 13 pM. This method showed a promising future for practical application in real water samples.

Keywords: Circular cleavage; DNAzyme; Entropy-driven amplification; Fluorescence; Uranyl.

MeSH terms

Biosensing Techniques*
DNA, Catalytic / chemistry*
DNA, Catalytic / metabolism
Entropy*
Fluorescence*
Nucleic Acid Amplification Techniques*
Uranium / analysis*

Substances

DNA, Catalytic
Uranium