Format

Send to

Choose Destination
Genome Biol. 2019 May 9;20(1):90. doi: 10.1186/s13059-019-1699-y.

CellTag Indexing: genetic barcode-based sample multiplexing for single-cell genomics.

Guo C1,2,3, Kong W1,2,3, Kamimoto K1,2,3, Rivera-Gonzalez GC1,2,3, Yang X1,2,3, Kirita Y2,4, Morris SA5,6,7.

Author information

1
Department of Developmental Biology, Washington University School of Medicine in St Louis, 660 S. Euclid Avenue, Campus Box 8103, St. Louis, MO, 63110, USA.
2
Department of Genetics, Washington University School of Medicine in St. Louis, 660 S. Euclid Avenue, Campus Box 8103, St. Louis, MO, 63110, USA.
3
Center of Regenerative Medicine, Washington University School of Medicine in St. Louis, 660 S. Euclid Avenue, Campus Box 8103, St. Louis, MO, 63110, USA.
4
Division of Nephrology, Department of Medicine, Washington University School of Medicine in St. Louis, 660 S. Euclid Avenue, Campus Box 8103, St. Louis, MO, 63110, USA.
5
Department of Developmental Biology, Washington University School of Medicine in St Louis, 660 S. Euclid Avenue, Campus Box 8103, St. Louis, MO, 63110, USA. s.morris@wustl.edu.
6
Department of Genetics, Washington University School of Medicine in St. Louis, 660 S. Euclid Avenue, Campus Box 8103, St. Louis, MO, 63110, USA. s.morris@wustl.edu.
7
Center of Regenerative Medicine, Washington University School of Medicine in St. Louis, 660 S. Euclid Avenue, Campus Box 8103, St. Louis, MO, 63110, USA. s.morris@wustl.edu.

Abstract

High-throughput single-cell assays increasingly require special consideration in experimental design, sample multiplexing, batch effect removal, and data interpretation. Here, we describe a lentiviral barcode-based multiplexing approach, CellTag Indexing, which uses predefined genetic barcodes that are heritable, enabling cell populations to be tagged, pooled, and tracked over time in the same experimental replicate. We demonstrate the utility of CellTag Indexing by sequencing transcriptomes using a variety of cell types, including long-term tracking of cell engraftment and differentiation in vivo. Together, this presents CellTag Indexing as a broadly applicable genetic multiplexing tool that is complementary with existing single-cell technologies.

Supplemental Content

Full text links

Icon for BioMed Central Icon for PubMed Central
Loading ...
Support Center