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J Gene Med. 2019 May 9:e3098. doi: 10.1002/jgm.3098. [Epub ahead of print]

Comparative analysis of transcriptomes between apical pulp derived cells from deciduous teeth and permanent teeth or dental pulp cells from exfoliated deciduous teeth.

Author information

1
Stomatological Hospital, Southern Medical University, Guangzhou, PR China.
2
Department of Stomatology, Guangzhou Women and Children's Medical Center, Guangzhou, PR China.

Abstract

BACKGROUND:

This study aimed to explore the differences between apical pulp derived cells of deciduous teeth (De-APDCs) and apical pulp derived cells of permanent teeth (P-APDCs)/dental pulp cells from exfoliated deciduous teeth (De-DPCs).

METHODS:

Three types of cells (De-DPCs, P-APDCs and De-APDCs) were cultured by tissue explant method. Transcriptome sequencing for the three types of cells was conducted and the differentially expressed genes (DEGs) between groups were selected. The potential biological functions and pathways enriched by the DEGs were analyzed.

RESULTS:

There were 35 up- and 21 down-regulated DEGs in P-APDC vs. De-APDC comparison group, and 328 up- and 976 down-regulated DEGs in De-APDC vs. De-DPC. The DEGs in P-APDC vs. De-APDC group were significantly enriched in BP terms associated with extracellular matrix organization, and regulation of cell proliferation and pathway of cell adhesion molecules. Additionally, the DEGs between De-APDC and De-DPC were involved in functions related to extracellular matrix organization, and regulation of bone remodeling, as well as pathway of ECM-receptor interaction.

CONCLUSIONS:

There were fewer differences between P-APDC and De-APDC compared with between De-APDC and De-DPC in view of the total number of DEGs. There existed differences in function related to extracellular matrix organization among the three cells. P-APDCs and De-APDC had different functions on cell proliferation and adhesion. De-APDCs may also act as a unique stem-cell resource for tissue engineering.

KEYWORDS:

apical pulp derived cells; deciduous teeth; dental pulp cells; exfoliated deciduous teeth; permanent teeth; transcriptome

PMID:
31070286
DOI:
10.1002/jgm.3098

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