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Oncoimmunology. 2019 Mar 22;8(6):e1588085. doi: 10.1080/2162402X.2019.1588085. eCollection 2019.

TCR sequencing analysis of cancer tissues and tumor draining lymph nodes in colorectal cancer patients.

Author information

1
Department of Medicine, The University of Chicago, Chicago, IL, USA.
2
Department of Surgery, Keio University School of Medicine, Tokyo, Japan.
3
Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama, Japan.
4
The PhD Program for Translational Medicine, College of Medical Science and Technology, Taipei Medical University and Academia Sinica, Taipei, Taiwan.
5
Department of Gastroenterological Surgery, Gastroenterological Center, Cancer Institute Hospital, Japanese Foundation for Cancer Research, Tokyo, Japan.
6
Cancer Precision Medicine Center, Japanese Foundation for Cancer Research, Tokyo, Japan.
7
Department of Gastroenterological, Breast and Endocrine Surgery, Yamaguchi University Graduate School of Medicine, Yamaguchi, Japan.
8
Department of Surgery, Hamamatsu University School of Medicine, Hamamatsu, Japan.
9
Department of Thoracic and Cardiovascular Surgery, Loyola University Medical Center, Maywood, IL, USA.

Abstract

Tumor draining lymph nodes (TDLNs) are located in the routes of lymphatic drainage from a primary tumor and have the highest risk of metastasis in various types of solid tumors. TDLNs are also considered as a tissue to activate the antitumor immunity, where antigen-specific effector T cells are generated. However, T cell receptor (TCR) repertoires in TDLNs have not been well characterized. We collected 23 colorectal cancer tumors with 203 lymph nodes with/without metastatic cancer cells (67 were metastasis-positive and the remaining 136 were metastasis-negative) and performed TCR sequencing. Metastasis-positive TDLNs showed a significantly lower TCR diversity and shared TCR clonotypes more frequently with primary tumor tissues compared to metastasis-negative TDLNs. Principal component analysis indicated that TDLNs with metastasis showed similar TCR repertoires. These findings suggest that cancer-reactive T cell clones could be enriched in the metastasis-positive TDLNs.

KEYWORDS:

T cell receptor; colorectal cancer; immunogenomics; immunotherapy; lymph node

PMID:
31069156
PMCID:
PMC6492974
[Available on 2020-03-22]
DOI:
10.1080/2162402X.2019.1588085

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