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Macromol Biosci. 2019 Jun;19(6):e1900020. doi: 10.1002/mabi.201900020. Epub 2019 May 8.

Precisely Controlled Delivery of Abaloparatide through Injectable Hydrogel to Promote Bone Regeneration.

Author information

1
Department of Orthopaedics and Traumatology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China.
2
Department of Spine Surgery and Institute for Orthopaedic Research, Shenzhen People's Hospital, Jinan University Second College of Medicine, The First Affiliated Hospital of Southern University of Science and Technology, Shenzhen, 518000, China.
3
Shanghai Key Laboratory for Bone and Joint Diseases, Shanghai Institute of Traumatology and Orthopaedics, Shanghai Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, 200000, China.
4
Department of Orthopaedics and Traumatology, The University of Hong Kong, Queen Mary Hospital, Pokfulam, Hong Kong.
5
School of Materials Science & Engineering, Tianjin University, Tianjin, 300000, China.
6
Shenzhen Institutes of Advanced Technology, Chinese Academy of Science, Shenzhen, 518000, China.

Abstract

Side-effects from allograft, limited bone stock, and site morbidity from autograft are the major challenges to traditional bone defect treatments. With the advance of tissue engineering, hydrogel injection therapy is introduced as an alternative treatment. Therapeutic drugs and growth factors can be carried by hydrogels and delivered to patients. Abaloparatide, as an analog of human recombinant parathyroid hormone protein (PTHrp) and an alternative to teriparatide, has been considered as a drug for treating postmenopausal osteoporosis since 2017. Since only limited cases of receiving abaloparatide with polymeric scaffolds have been reported, the effects of abaloparatide on pre-osteoblast MC3T3-E1 are investigated in this study. It is found that in vitro abaloparatide treatment can promote pre-osteoblast MC3T3-E1 cells' viability, differentiation, and mineralization significantly. For the drug delivery system, 3D porous structure of the methacrylated gelatin (GelMA) hydrogel is found effective for prolonging the release of abaloparatide (more than 10 days). Therefore, injectable photo-crosslinked GelMA hydrogel is used in this study to prolong the release of abaloparatide and to promote healing of defected bones in rats. Overall, data collected in this study show no contradiction and imply that Abaloparatide-loaded GelMA hydrogel is effective in stimulating bone regeneration.

KEYWORDS:

abaloparatide; bone regeneration; hydrogel; methacrylated gelatin; parathyroid hormone

PMID:
31066995
DOI:
10.1002/mabi.201900020

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