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Plant Cell Rep. 2019 Aug;38(8):981-990. doi: 10.1007/s00299-019-02419-9. Epub 2019 May 7.

Altered levels of mitochondrial NFS1 affect cellular Fe and S contents in plants.

Author information

1
Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI-CONICET), Universidad Nacional de Rosario, 2000, Rosario, Argentina.
2
Instituto de Investigaciones Biotecnológicas, IIB-INTECH, CONICET-UNSAM, Chascomús, Argentina.
3
Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI-CONICET), Universidad Nacional de Rosario, 2000, Rosario, Argentina. gomezcasati@cefobi-conicet.gov.ar.

Abstract

The ISC Fe-S cluster biosynthetic pathway would play a key role in the regulation of iron and sulfur homeostasis in plants. The Arabidopsis thaliana mitochondrial cysteine desulfurase AtNFS1 has an essential role in cellular ISC Fe-S cluster assembly, and this pathway is one of the main sinks for iron (Fe) and sulfur (S) in the plant. In different plant species it has been reported a close relationship between Fe and S metabolisms; however, the regulation of both nutrient homeostasis is not fully understood. In this study, we have characterized AtNFS1 overexpressing and knockdown mutant Arabidopsis plants. Plants showed alterations in the ISC Fe-S biosynthetic pathway genes and in the activity of Fe-S enzymes. Genes involved in Fe and S uptakes, assimilation, and regulation were up-regulated in overexpressing plants and down-regulated in knockdown plants. Furthermore, the plant nutritional status in different tissues was in accordance with those gene activities: overexpressing lines accumulated increased amounts of Fe and S and mutant plant had lower contents of S. In summary, our results suggest that the ISC Fe-S cluster biosynthetic pathway plays a crucial role in the homeostasis of Fe and S in plants, and that it may be important in their regulation.

KEYWORDS:

Cysteine desulfurase; Fe–S clusters; Iron; Sulfur

PMID:
31065779
DOI:
10.1007/s00299-019-02419-9
[Indexed for MEDLINE]

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