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Int J Mol Sci. 2019 May 6;20(9). pii: E2219. doi: 10.3390/ijms20092219.

PgMYB2, a MeJA-Responsive Transcription Factor, Positively Regulates the Dammarenediol Synthase Gene Expression in Panax Ginseng.

Author information

1
Department of Biochemistry and Molecular Biology, School of Life Sciences, Central South University, Changsha 410008, China. lt1994@csu.edu.cn.
2
Department of Biochemistry and Molecular Biology, School of Life Sciences, Central South University, Changsha 410008, China. tiaooul96@163.com.
3
School of Stomatology of Changsha Medical University, Changsha 410006, China. tiaooul96@163.com.
4
Department of Biochemistry and Molecular Biology, School of Life Sciences, Central South University, Changsha 410008, China. gxq199x@163.com.
5
Department of Biochemistry and Molecular Biology, School of Life Sciences, Central South University, Changsha 410008, China. zx13618463547@163.com.
6
School of Stomatology of Changsha Medical University, Changsha 410006, China. csyxyzdh@163.com.
7
Department of Biochemistry and Molecular Biology, School of Life Sciences, Central South University, Changsha 410008, China. nilabotdu@yahoo.com.
8
Department of Biochemistry and Molecular Biology, School of Life Sciences, Central South University, Changsha 410008, China. ligui20061029@126.com.
9
Department of Biochemistry and Molecular Biology, School of Life Sciences, Central South University, Changsha 410008, China. zhang1045242781@126.com.
10
College of Chemistry and Chemical Engineering, Hunan Institute of Engineering, Xiangtan 411104, China. zhangru2002@126.com.
11
Department of Biochemistry and Molecular Biology, School of Life Sciences, Central South University, Changsha 410008, China. luozhiyong@csu.edu.cn.

Abstract

The MYB transcription factor family members have been reported to play different roles in plant growth regulation, defense response, and secondary metabolism. However, MYB gene expression has not been reported in Panax ginseng. In this study, we isolated a gene from ginseng adventitious root, PgMYB2, which encodes an R2R3-MYB protein. Subcellular localization revealed that PgMYB2 protein was exclusively detected in the nucleus of Allium cepa epidermis. The highest expression level of PgMYB2 was found in ginseng root and it was significantly induced by plant hormones methyl jasmonate (MeJA). Furthermore, the binding interaction between PgMYB2 protein and the promoter of dammarenediol synthase (DDS) was found in the yeast strain Y1H Gold. Moreover, the electrophoretic mobility shift assay (EMSA) identified the binding site of the interaction and the results of transiently overexpressing PgMYB2 in plants also illustrated that it may positively regulate the expression of PgDDS. Based on the key role of PgDDS gene in ginsenoside synthesis, it is reasonable to believe that this report will be helpful for the future studies on the MYB family in P. ginseng and ultimately improving the ginsenoside production through genetic and metabolic engineering.

KEYWORDS:

MYB transcription factor; Panax ginseng; dammarenediol synthase; gene expression; ginsenoside; methyl jasmonate

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