Send to

Choose Destination
Vet Clin Pathol. 2019 Jun;48(2):282-286. doi: 10.1111/vcp.12732. Epub 2019 May 6.

The utility of intraoperative impression smear cytology of intracranial granular cell tumors: Three cases.

Author information

Department of Veterinary Clinical Medicine, The University of Illinois, Urbana, Illinois.
Department of Pathobiology, The University of Illinois, Urbana, Illinois.
Department of Neuroscience, Ipsen Pharmaceutical, Paris, France.
MedVet Medical and Cancer Centers for Pets, Worthington, Ohio.


Two adult male dogs (a 7-year-old shorthaired Chihuahua and 14-year-old Shih Tzu) and one adult female dog (a 9-year-old Maltese) presented for evaluation of new-onset seizure activity. Magnetic resonance imaging of the brain demonstrated a large, poorly marginated T2-weighted hyperintense, and strong contrast enhancing extra-axial mass in each case. A surgical biopsy for histopathologic evaluation was elected in all cases, and intraoperative impression smears were successfully obtained. Intraoperative cytology identified a homogenous population of round to polygonal cells with central to eccentric nuclei, coarse chromatin, and variably amphophilic to eosinophilic granular cytoplasm. Cytologic findings led to a suspected diagnosis of granular cell tumor (GCT) in all cases. Histopathologic review identified a densely cellular, unencapsulated neoplastic mass comprised of sheets of large round to polygonal cells with abundant eosinophilic cytoplasm containing numerous eosinophilic intracytoplasmic granules, confirming the diagnosis of GCT in all cases. The cases reported here are unique in that they reveal an accurate intraoperative cytologic diagnosis of a rare canine central nervous system neoplasm. Intraoperative cytology of the intracranial masses could provide clinicians with important and quick diagnostic and prognostic information; therefore, expediting decisions made intraoperatively. Further research is warranted to determine the diagnostic accuracy of intraoperative cytology for neoplasia in veterinary patients.


brain; canine; granular cell tumor; impression; neoplasia

[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Wiley
Loading ...
Support Center