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Proc Natl Acad Sci U S A. 2019 Jun 4;116(23):11207-11212. doi: 10.1073/pnas.1902174116. Epub 2019 May 6.

Chemical evidence for the use of multiple psychotropic plants in a 1,000-year-old ritual bundle from South America.

Author information

Department of Anatomy, University of Otago, Dunedin 9016, New Zealand;
Archaeological Research Facility, University of California, Berkeley, CA 94720.
Instituto de Investigaciones Antropológicas y Arqueológicas, Universidad Mayor de San Andrés, La Paz, Bolivia.
Immunalysis Corporation, Pomona, CA 91767.
Department of Anthropology, The Pennsylvania State University, University Park, PA 16802


Over several millennia, various native plant species in South America have been used for their healing and psychoactive properties. Chemical analysis of archaeological artifacts provides an opportunity to study the use of psychoactive plants in the past and to better understand ancient botanical knowledge systems. Liquid chromatography tandem mass spectrometry (LC-MS/MS) was used to analyze organic residues from a ritual bundle, radiocarbon dated to approximately 1,000 C.E., recovered from archaeological excavations in a rock shelter located in the Lípez Altiplano of southwestern Bolivia. The site is located at an elevation of ∼3,900 m above sea level and contains evidence of intermittent human occupations during the last 4,000 years. Chemical traces of bufotenine, dimethyltryptamine, harmine, and cocaine, including its degradation product benzoylecgonine, were identified, suggesting that at least three plants containing these compounds were part of the shamanic paraphernalia dating back 1,000 years ago, the largest number of compounds recovered from a single artifact from this area of the world, to date. This is also a documented case of a ritual bundle containing both harmine and dimethyltryptamine, the two primary ingredients of ayahuasca. The presence of multiple plants that come from disparate and distant ecological areas in South America suggests that hallucinogenic plants moved across significant distances and that an intricate botanical knowledge was intrinsic to pre-Columbian ritual practices.


archaeometry; exchange; hallucinogen; liquid chromatography mass spectrometry; shamanism

[Available on 2019-11-06]

Conflict of interest statement

The authors declare no conflict of interest.

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