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Curr Pharm Biotechnol. 2019;20(6):517-524. doi: 10.2174/1389201020666190506124548.

Does Nimodipine, a Selective Calcium Channel Blocker, Impair Chondrocyte Proliferation or Damage Extracellular Matrix Structures?

Author information

1
Department of Neurosurgery, Corlu Reyap Hospital, Istanbul Rumeli University, 59680, Tekirdag, Turkey.
2
Department of Medical Pharmacology, Istanbul Medipol University School of Medicine, 34810, Istanbul, Turkey.
3
Department of Neurosurgery, Namik Kemal University School of Medicine, 59100, Tekirdag, Turkey.
4
Department of Orthopedic and Traumatology, Abant Izzet Basal University School of Medicine, 14000, Bolu, Turkey.
5
Department of Molecular Biology and Genetics, Faculty of Arts and Sciences, Namik Kemal University, 59100, Tekirdag, Turkey.

Abstract

BACKGROUND:

The study aimed to investigate the effects of the active ingredient, nimodipine, on chondrocyte proliferation and extracellular matrix (ECM) structures in cartilage tissue cells.

METHODS:

Chondrocyte cultures were prepared from tissues resected via surgical operations. Nimodipine was then applied to these cultures and molecular analysis was performed. The data obtained were statistically calculated.

RESULTS:

Both, the results of the (3-(4,5 dimethylthiazol2-yl)-2,5-diphenyltetrazolium (MTT) assay and the fluorescence microscope analysis [a membrane permeability test carried out with acridine orange/ propidium iodide staining (AO/PI)] confirmed that the active ingredient, nimodipine, negatively affects the cell cultures.

CONCLUSION:

Nimodipine was reported to suppress cellular proliferation; chondroadherin (CHAD) and hypoxia-inducible factor-1 alpha (HIF-1α) expression thus decreased by 2.4 and 1.7 times, respectively, at 24 hrs when compared to the control group (p < 0.05). Furthermore, type II collagen (COL2A1) expression was not detected (p < 0.05). The risk that a drug prescribed by a clinician in an innocuous manner to treat a patient by relieving the symptoms of a disease may affect the proliferation, differentiation, and viability of other cells and/or tissues at the molecular level, beyond its known side effects or adverse events, should not be forgotten.

KEYWORDS:

Chondroadherin; chondrocyte; hypoxia-inducible factor-1 alpha; matrix structures; nimodipine; type II collagen.

PMID:
31057106
PMCID:
PMC6751346
DOI:
10.2174/1389201020666190506124548
[Indexed for MEDLINE]
Free PMC Article

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