Format

Send to

Choose Destination
Theriogenology. 2019 Jul 15;133:22-28. doi: 10.1016/j.theriogenology.2019.04.029. Epub 2019 Apr 26.

Changes in sperm parameters of sex-reversed female mandarin fish Siniperca chuatsi during cryopreservation process.

Author information

1
Guangdong Provincial Key Laboratory for Aquatic Economic Animals, Institute of Aquatic Economic Animals, School of Life Sciences, Sun Yat-sen University, Guangzhou, 510006, China; School of Life Sciences, Sun Yat-sen University, Guangzhou, 510006, China; Guangdong Provincial Engineering Technology Research Center for Healthy Breeding of Important Economic Fish, Guangzhou, 510006, China.
2
Guangdong Provincial Key Laboratory for Aquatic Economic Animals, Institute of Aquatic Economic Animals, School of Life Sciences, Sun Yat-sen University, Guangzhou, 510006, China; School of Life Sciences, Sun Yat-sen University, Guangzhou, 510006, China; Guangdong Provincial Engineering Technology Research Center for Healthy Breeding of Important Economic Fish, Guangzhou, 510006, China. Electronic address: liguif@mail.sysu.edu.cn.

Abstract

This works studies the physiological characteristics (sperm motility characteristics) and biochemical characteristics (DNA damage, enzymes activities and fertilization ability) of fresh and freeze-thawed semen of sex-reversed female mandarin fish Siniperca chuatsi (n = 30) obtained with the application of 17α-methyl testosterone for sex reversal. The fresh sperm motility and fertilization rate of sex-reversed females were about 83% and 70% respectively which had no significant difference with normal males (p > 0.05). Except for the value of DNA damage, other values of sperm motility, related enzymes activities and fertilization rate of sex-reversed female sperm declined after a process of cryopreservation (p < 0.05). But the frozen sperm can still get nearly 60% of the fertilization rate. This study identified the physiological and biochemical characteristics of the fresh and cryopreserved sperm from sex-reversed female mandarin fish, and the sex-reversed female spermatozoa can be used for actual production.

KEYWORDS:

Biological characteristics; Cryopreservation; Sex-reversed females; Siniperca chuatsi; Sperm

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center