Send to

Choose Destination
FASEB J. 2019 Aug;33(8):8892-8904. doi: 10.1096/fj.201802626R. Epub 2019 May 3.

Two pools of IRE1α in cardiac and skeletal muscle cells.

Author information

Department of Biochemistry, University of Alberta, Edmonton, Alberta, Canada.
National "111" Center for Cellular Regulation and Molecular Pharmaceutics, Hubei University of Technology, Wuhan, China.
Division of Pharmacology, Vanderbilt University School of Medicine, Nashville, Tennessee, USA.
Department of Physiology and Pharmacology, Libin Cardiovascular Institute of Alberta, Cumming School of Medicine, University of Calgary, Calgary, Alberta, Canada.
Department of Physiology, University of Alberta, Edmonton, Alberta, Canada; and.
School of Human Nutrition, McGill University, Ste. Anne de Bellevue, Quebec, Canada.


The endoplasmic reticulum (ER) plays a central role in cellular stress responses via mobilization of ER stress coping responses, such as the unfolded protein response (UPR). The inositol-requiring 1α (IRE1α) is an ER stress sensor and component of the UPR. Muscle cells also have a well-developed and highly subspecialized membrane network of smooth ER called the sarcoplasmic reticulum (SR) surrounding myofibrils and specialized for Ca2+ storage, release, and uptake to control muscle excitation-contraction coupling. Here, we describe 2 distinct pools of IRE1α in cardiac and skeletal muscle cells, one localized at the perinuclear ER and the other at the junctional SR. We discovered that, at the junctional SR, calsequestrin binds to the ER luminal domain of IRE1α, inhibiting its dimerization. This novel interaction of IRE1α with calsequestrin, one of the highly abundant Ca2+ handling proteins at the junctional SR, provides new insights into the regulation of stress coping responses in muscle cells.-Wang, Q., Groenendyk, J., Paskevicius, T., Qin, W., Kor, K. C., Liu, Y., Hiess, F., Knollmann, B. C., Chen, S. R. W., Tang, J., Chen, X.-Z., Agellon, L. B., Michalak, M. Two pools of IRE1α in cardiac and skeletal muscle cells.


ER stress; calcium; calsequestrin; sarcoplasmic reticulum

[Available on 2020-05-03]

Supplemental Content

Full text links

Icon for Atypon
Loading ...
Support Center